NOD.Scid过继性1型糖尿病小鼠模型的建立  

The establishment of adoptive transfer model of type 1 diabetes in NOD.Scid mice

作　　者：冯皓[1] 郭葆玉[1] 张淑英[1] 

机构地区：[1]第二军医大学药学院生化药学教研室,上海200433

出　　处：《中国免疫学杂志》2005年第8期619-621,共3页Chinese Journal of Immunology

基　　金：国家自然科学基金项目(30371349)资助

摘　　要：目的:建立能反应自身免疫特点的1型糖尿病动物模型,为进一步研究该病发病机理奠定基础.方法:将16只NOD.Scid小鼠随机分为两组,实验组小鼠腹腔注射发病NOD小鼠脾细胞,对照组注射未发病NOD小鼠脾细胞;每日测定血糖和体重;当出现重度糖尿病临床表现时处死,其余小鼠细胞过继后10周处死,经组织学观察和细胞因子ELISA测定.结果:实验组小鼠发病率为8/8,对照组为0/8;胰岛炎性积分分别为2.5±0.2,0;实验组IL-2、IL-10、IFN-γ水平分别为60.7 pg/ml、15.5 pg/ml、20.2 ng/ml,对照组为2.4 pg/ml、17.5 pg/ml、3.2 ng/ml.结论:在NOD.Scid小鼠体内一次性过继发病NOD小鼠脾细胞后5～10周可成功建立1型糖尿病的小鼠模型.Objective：To establish adoptive transfer model mice ot type 1 diabetes for investigating the pathogenesis ot type 1 diabetes. Methods： 16 NOD. Scid mice were randomly divided into two groups. One was injected i. p, with splenoeytes which were obtained from a diabetic NOD mouse and the other was injected with splenoeytes obtained from a normal one. The blood glucose levels and weight were detected daily. The mice were killed when they had significant clinic appearance or 10 weeks after cell injection, followed by pathologic studies and cytokine assays. Results： The incidence of adoption group was 8/8 wbereas that of control group was 0/8. The pathology scores of 2 groups were 2.5±0.2,0 separately. The amounts of IL-2, IL- 10, IFN-γ were 60.7 pg/ml, 15.5 pg/ml and 20.2 ng/ml respectively in adoption group, and those in control group were 2.4 pg/ml, 17.5 pg/ml,3.2 ng/ml. Conelusion： Model rniee of type 1 diabetes can be established in NOD. Scid mice during the 5-10 week after adoptive transfer of splenoeytes of diabetic NOD mice.

关 键 词：糖尿病 动物模型 NOD 

分 类 号：R587.103[医药卫生—内分泌]