TRAIL对HBV感染的免疫监视效应研究  

Immune surveillance effect of TRAIL on the HBV infection

作　　者：韩丽辉[1] 孙汶生[1] 贾晓青[2] 张利宁[1] 曹英林[1] 高立芬[1] 朱法良[1] 郭春[1] 

机构地区：[1]山东大学医学院免疫学研究所,山东济南250012 [2]山东大学齐鲁医院消化内科,山东济南250012

出　　处：《山东大学学报（医学版）》2005年第7期553-556,共4页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助项目(30128023;30371342);教育部留学回国人员科研启动基金资助项目(2004.527);山东省基金资助项目(2004BS0301)。

摘　　要：目的:探索新型肿瘤坏死因子(TNF)相关的凋亡诱导配体(TRAIL)对乙型肝炎病毒(HBV)感染后的免疫监视效应。方法:应用酶联免疫吸咐(ELISA)和噻唑蓝(MTT)比色法及流式细胞术检测52例HBV感染者和24例健康对照者血清中可溶性TRAIL(sTRAIL)的表达,在转染HBV全基因组并稳定表达病毒抗原的HepG2.2.15肝癌细胞系研究TRAIL在γ鄄干扰素(IFN鄄γ)的作用下对HepG2.2.15细胞的生长抑制效应和凋亡诱导效应。结果:HBV感染者的外周血sTRAIL的表达量明显高于正常对照组(P<0.001)。各组HBV感染者sTRAIL的表达量与丙氨酸氨基转换酶(ALT)的值呈正相关。而细胞学的研究结果显示,在IFN鄄γ的协同作用下,TRAIL能够显著抑制HepG2.2.15细胞的恶性增殖,并能够显著诱导HBV相关肿瘤细胞发生凋亡。结论:TRAIL是参与到HBV感染后所致疾病中的一个重要的免疫监视分子,TRAIL的作用效应极有可能直接影响疾病的预后与转归。Objective：To explore the immune surveillance effect of TRAIL, a new tumor necrosis factor-related apoptosis inducing ligand on the HBV related diseases. Methods： The expression of sTRAIL in 52 HBV infected patients and 24 healthy controls, the growth inhibitory effect and apoptosis inducing effect of TRAIL and IFN-У, in HepG2.2.15 hepatocarcinomatous cell line transfecting HBV were studied by ELISA, MTT and FCM assay, respectively. Results： The expression of sTRAIL in the HBV infected group was significantly higher than that in the healthy control group （P ＜ 0.001）. In the HBV infected group, the expression level of sTRAIL was positively related to the ALT value. Cellular study showed that with the cooperation of IFN-У, TRAIL could significantly inhibit the growth and induce apoptosis of the HBV related HepG2.2.15 cells. Conclusion： TRAIL is an important immune surveillance molecule that probably plays a key role in the prognosis of the HBV related disease.

关 键 词：肝炎病毒 乙型 细胞凋亡 配体 免疫学监视 

分 类 号：R392.12[医药卫生—免疫学]