重组人白细胞介素18对小鼠骨髓细胞移植后急性移植物抗宿主病的影响  

Inhibitory effect of rhIL-18 on murine aGVHD following allogeneic bone marrow transplantation

作　　者：刘兴田[1] 张利宁[1] 王玉坤[2] 王晓燕[1] 高丽芬[1] 王群[1] 李娜[1] 刘军莉[1] 

机构地区：[1]山东大学医学院免疫学研究所,山东济南250012 [2]山东大学齐鲁医院皮肤科,山东济南250012

出　　处：《山东大学学报（医学版）》2005年第7期557-560,共4页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助课题(30271247和30371352)。

摘　　要：目的:探讨重组人白细胞介素18(rhIL-18)对小鼠异基因骨髓移植后急性移植物抗宿主病(aGVHD)的影响,为rhIL-18用于临床移植免疫提供理论基础。方法:受体鼠(BALB/c)接受9.0Gy剂量的X-射线照射之后,通过其尾静脉注射供体鼠(C57BL/6)的骨髓细胞和脾细胞,并于移植的-2^+2d腹腔注射rhIL-18。移植后观察受体鼠的临床症状和存活状况,同时检测其肝脏、结肠和耳朵的病理变化。结果:移植后aGVHD期内,用rhIL-18治疗的受体鼠GVHD症状积分较未接受rhIL-18治疗的受体鼠低(P<0.05),肝脏和结肠的病理变化轻(P<0.05),短期存活时间延长(P<0.05),但对长期生存率无明显影响。结论:受体鼠早期接受rhIL-18治疗,可在移植后aGVHD期内降低aGVHD的病变程度,为rhIL-18在临床上预防和治疗aGVHD提供了实验依据。Objective： To explore the effect of recombinant-human interleukin-18 （rhIL-18） on murine acute graft versus host disease （aGVHD） following allogeneic bone marrow cells and spleen cells transplantation. Methods：After lethally irradiated with X-ray, bone marrow and spleen cells from donor C57BL/6 mice were injected into recipient BALB/c mice via tail vein on 0 day and rhIL-18 intraperioneallyduring -2～＋2 days. The clinical symptoms and survival rates of mice in each group were observed during acute phase of GVHD following transplantation, and the pathological changes of mice＇liver, colon and ear were examined by routine pathological methods. Results： The mice in rhIL-18 treatment group had less clinical symptoms scores （P ＜ 0.05）, slighter pathological changes of liver and colon （P ＜ 0.05） and high short-term survival rate than the mice in rhIL-18 untreatment group during theeracute GVHD following transplantation （P ＜ 0.05）. But there was no difference in long-term survival rates between IL-18-treatment group and rhIL-18-untreatment group. Conclusion： The early administration of rhIL-18 in recipients can reduce the severity of aGVHD but has no significant effect on their long-term survival rates. These provide experimental data for clinical using of rhIL-18 in future.

关 键 词：白细胞介素18 骨髓移植 移植物抗宿主病 小鼠 近交BALB C 

分 类 号：R392.11[医药卫生—免疫学] R-322[医药卫生—基础医学]