去甲万古霉素载药涤纶血管材料体外抗菌活性测定  

Measurement of in vitro antibacterial activity of norvancomycin-bonded Dacron prostheses

作　　者：余维平[1] 景在平[1] 包俊敏[1] 赵志青[1] 赵珺[1] 冯翔[1] 陆清声[1] 

机构地区：[1]第二军医大学附属长海医院血管外科,上海200433

出　　处：《中国现代普通外科进展》2005年第4期231-233,共3页Chinese Journal of Current Advances in General Surgery

基　　金：军队杰出人才基金项目(98J005);上海市卫生系统"百人计划"资助项目(97BR047)

摘　　要：目的:评价去甲万古霉素(NV)载药涤纶血管材料体外抗菌活性。方法:血管材料剪成直径6mm圆形,分别制成空白涤纶材料和载药(NV)涤纶材料,分别于0,7,14,28d取样观察体外缓释性能。选用耐甲氧西林表皮葡萄球菌(MRSE)和耐甲氧西林金黄色葡萄球菌(MRSA)作为试验菌,分别观察空白和载药涤纶血管材料0,7,14,28d和快慢相的抗菌效果。结果:两种细菌0,7,14,28d载药涤纶材料细菌混合培养液的菌落数显著少于空白涤纶材料细菌混合培养液(P均<0.001)。结论:体外试验证实载NV涤纶材料MRSE和MRSA具有抗菌作用,且可持续维持28d,提示该载药材料具有持久抗菌活性,可用于制备抗感染血管移植物。Objective：To evaiuate in vitro antibacterial activity of norvancomycin-bonded Dacron prostheses.Methods：With the use of methicillin-restistant S.epidermidis（MRSE） and methicillin-resistant S.aureus（MRSA）as test organisms.culture,culture of the mixture of a piece of 6-mm-diameter round prostheses（after soaking and shaking in PBS for 0,7,14 and 28 days）,10ml broth containing 0.5ml bacterial suspension was performed toassess the quantity of bacteria grown,the colony-forming units（CFU） of staphylococcus were then plate-counted after culturing.Statistical analysisi was completed using the paired t-test.Results：Norvancomycin-bonded Dacron prostheses produced significantly lower colony counts compared with unprotected Dacron prostheses for the two staphylococcus at 0,7,14 and 28 days.Conclusion：Norvancomycin-bonded Dacron prostheses exhibits persistent in vitro antibacterial activity against the two bacteria for up to 28 days.These results suggeste the norvancomycin-bonded Dacron prostheses as the promising infection-resistant vascular graft.

关 键 词：涤纶血管材料 去甲万古霉素 体外抗菌活性 

分 类 号：R543[医药卫生—心血管疾病]