用骨髓脂肪建立脂肪栓塞综合征动物模型  

THE EXPERIMENTAL REPRODUCTION OF AN ANIMAL MODEL OF FAT EM- BOLISM SYNDROME WITH BONE MARROW FAT

作　　者：张伯勋[1] 滕青山[1] 马承宣[1] 朱新华[1] 李刚[1] 

机构地区：[1]解放军总医院

出　　处：《解放军医学杂志》1995年第1期52-54,共3页Medical Journal of Chinese People's Liberation Army

摘　　要：观察脂肪栓塞综合征动物模型表明：①犬的静脉血凝块冰冻切片油红Ｏ染色，可以找到桔红色的脂滴，并可计算出脂滴数量和大小；②取实验犬的肺组织冰冻切片油红Ｏ染色，也可看到大量的桔红色脂滴沉着于肺组织内；③用气相色谱法检测血浆游离脂肪酸时，发现静脉注射骨髓脂肪后游离脂肪酸明显升高；④犬的动脉血氧分压下降，当降至７．９９±０．３０ｋＰａ时，犬出现呼吸困难等症状。上述结果说明，用骨髓脂肪静脉注射建立脂肪栓塞综合征的动物模型是可靠的。An experimental animal model for fat embolism syndrome had been reproduced successful-ly with intravenous injection of 0. 7m1/kg of homologous bone marrow fat. Results as follows :(1)Manyorange-colored fat droplets were found in the frozen sections of venous blood clot stained with oil redstain. The number and diameter of fat droplets were calculated; (2)A lot of orange-colored fat dropletsdeposited in lung tissue were also found in frozen sections of lung tissue stained with oil red stain;(3)Asharp increase of free fatty acid in serum was found after intravenous injection of bone marrow fat withgas chromatography; (4 )A decrease of PaO2 was demonstrated, and shortness of breath Occurred whenthe PaO2 declined to 7.99±0.30kPa(60mmHg). The above results showed that fat embolism syndrome could be reproduced in dogs with intravenousinjection of bone marrow fat globules.

关 键 词：骨髓脂肪 脂肪栓塞综合征 动物模型 

分 类 号：R589.202[医药卫生—内分泌]