流行性出血热灭活疫苗免疫小鼠细胞免疫应答状况的研究  

THE CELLULA IMMUNE RESPONSES IN MICE IMMUNIZED WITH AN INACTI-VATED VACCINE AGAINST EPIDEMIC HEMORRHAGIC FEVER

作　　者：陈龙邦[1] 王彦宏 黄永成[1] 宋干 

机构地区：[1]南京南京军区南京总医院

出　　处：《解放军医学杂志》1995年第1期13-15,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金

摘　　要：为阐明流行性出血热（ＥＨＦ）灭活疫苗的细胞免疫保护性机制，对地鼠肾细胞培养灭活疫苗免疫小鼠的脾细胞增殖，产生白细胞介素－２（ＩＬ－２）和干扰素（ＩＦＮ）的能力进行了研究。结果表明，疫苗免疫小鼠脾细胞对ＣｏｎＡ及ＥＨＦ病毒抗原的刺激指数均显著高于正常小鼠。两组小鼠脾细胞在ＣｏｎＡ刺激下产生ＩＬ－２和ＩＦＮ，的水平无显著差异，但免疫小鼠受ＥＨＦ病毒抗原刺激后产生的ＩＬ－２水平明显高于正常对照组；１５只免疫小鼠有９只对病毒抗原的刺激产生ＩＦＮ反应；免疫小鼠血清特异性抗体阳转率为９３．３％。上述结果对于评价ＥＨＦ灭活疫苗的免疫效果具有重要价值。To elucidate the protective mechanism of cellular immunityof inactivated vaccine againstepidemic hemorrhagic fever (EHF), we assayed the lymphocyte blastogenesis, responses of interleukin-2(IL-2)and immune interferon (IFNr ) in mice immunized with inactivated vaccine against EHF cultivatedin golden hamster kidney cells. It was found that the proliferating indices of spleen cells stimulated withEHF virus antigen and Con A in immunized mice were significantly higher than those in normal mice. Nodifference in levels of IL-2 and IFNr produced by spleen cells cocultured with Con A was observed in bothimmunized group and controls, however, the level of IL-2 stimulated with EHF virus antigen in the im-munized mice were obviously higher than that in the controls. 9 of 15 immunized mice produced an IFNrresponse to stimulation of EHF virus antigen. The serum conversion rate of IFA antibodies was 93.3%in the immunized. The above results are valuable in evaluating the immune effects of the inactivated vac-cine against EHF.

关 键 词：流行性出血热 灭活疫苗 细胞免疫 淋巴细胞 

分 类 号：R512.803[医药卫生—内科学]