卡维地洛对大鼠心室肌细胞I_(to)电流影响的实验研究  

Study of effects of carvedilol on transient outwardpotassium current (I_(to)) of ventricular myocytes of rats

作　　者：曹艳杰[1] 王海昌[1] 杜俊杰[2] 张荣庆[1] 张清[1] 贾国良[1] 招明高[3] 

机构地区：[1]第四军医大学西京医院心脏内科 [2]第四军医大学西京医院创伤矫形外科研究所 [3]第四军医大学基础部药理学教研室,陕西西安710032

出　　处：《中国现代医学杂志》2005年第15期2268-2270,共3页China Journal of Modern Medicine

摘　　要：目的探讨卡维地洛对大鼠心室肌细胞瞬时外向钾电流(Ito)的作用。方法采用胶原酶酶解法分离得到大鼠单心室肌细胞;采用膜片钳全细胞技术记录灌流卡维地洛前后Ito电流的变化。结果灌流0.1mM及1.0mM卡维地洛后,Ito电流密度分别为(pA/pF):(5.06±1.98),(3.91±0.66),P<0.05;不同去极化电压水平Ito电流值也有明显减小,I-V曲线向下方移位。结论卡维地洛对大鼠心室肌细胞Ito电流具有抑制作用,此抑制作用呈浓度依赖趋势。[Objective] To explore the effects of carvedilol on transient outward potassium current （Ito） of ventricular cardiomyocytes of rats. [Methods] Rat cardiomyocytes were isolated with collagenase to acquire single ventricular myocytes. Ito current was recorded using whole-cell recording patch clamp technique. The influences of carvedilol on Ito were studied. [Results] Ito current density （pA/pF） was： （5.06±1.98）, （3.91±0.66）,respectively, when 0.1 mM, 1.0 mM carvedilol was added to extracellular bath solution （P 〈0.05）. Current-voltage relationship （I-V） curve was shifted downward. [Conclusions] Carvedilol has significant inhibitive effects on Ito. The inhibitive effects are more orominent in higer concentrations than in lower concentratons.

关 键 词：卡维地洛 Ito电流 膜片钳技术 

分 类 号：R331.38[医药卫生—人体生理学] R541.7[医药卫生—基础医学]