细胞坏死和凋亡（Apoptosis）DNA状态的比较  

COMPARISON OF DNA STRAND BREAKS BETWEEN APOPTOTIC AND NECROTIC CELLS

作　　者：牛建昭[1] 魏育林[1] 曹炜[1] 

机构地区：[1]北京中医药大学组织学胚胎学教研室,中日友好医院临床医学研究所中西医结合研究室

出　　处：《解剖学报》1995年第2期142-145,共4页Acta Anatomica Sinica

摘　　要：用琼脂糖凝胶电泳法、非放射性缺口翻译标记法（ｎｉｃｋｔｒａｎｓｌａｔｉｏｎ，ＮＴ）和末端脱氧核苷酸转移酶（ｔｅｒｍｉｎａｌｄｅｏｘｙｎｕｃｌｅｏｔｉｄｙｌｔｒａｎｓｆｅｒａｓｅ，ＴｄＴ）标记法研究细胞凋亡（ａｐｏｐｔｏｓｉｓ）和坏死时细胞总ＤＮＡ的ＤＮＡ断裂（ＤＮＡｓｔｒａｎｄｂｒｅａｋｓ，ＤＳＢ）状态。细胞凋亡标本取自皮下注射氢化可的松（１０ｍｇ／１００ｇ体重）的大鼠胸腺；坏死标本采用ＣＣｌ＿４（１００μ１／１００ｇ体重）的大鼠肝脏。结果表明：经激素处理的胸腺组织ＤＮＡ在琼脂糖凝胶电泳呈现清晰、规则的“梯子”状（ｌａｄｄｅｒ）带纹，而ＣＣｌ＿４处理后的肝脏组织ＤＮＡ则呈现不规则的带纹。缺口翻译标记时，凋亡和坏死组织的ＤＮＡ显色无差别，但ＴｄＴ末端标记时，坏死组织的ＤＮＡ显色较凋亡组织的ＤＮＡ显色速度快，着色深。以上结果提示：１．细胞凋亡和坏死时均存在ＤＳＢ，但ＤＳＢ类型不同。２．凋亡时ＤＮＡ降解成寡聚核小体而出现规则的“ｌａｄｄｅｒ”，坏死时ＤＮＡ降解成长短不等的片段。３．ＴｄＴ末端标记法显示，细胞凋亡ＤＳＢ显色特性不同于坏死。Cell death is currently divided into two catogories,apoptosis necrosis. We investigated DNA strand breaks(DSB)in apoptotic and necrotic cells by gel electrophoresis and labeling of extracted DNA by nonradioactive nick translation and TdT tailing reaction,As models of apoptosis and necrosis,rat thymus with an i.p.injection of hydrocortisone(10 mg/100g BW)and rat livers treated with CCl4(1 00μl/1 00 g BW)were used respectively. The DNA from the hydrocortisontreated thymus revealed a typical ladder from of DNA fragments in gel electrophoresis,When the same amount of DNA from the CCl4-treated liver was analyzed by gel electrophoresis,nonregular pattern of banding was detected.The labeling density of the extracted DNAs by nick translation was at the same level,while the DNA from the necrotic tissue was labeled by TdT was much denser than that of from the apoptotic tissue.These results indicate that 1.Although DSB occured both in apoptosis and in necrosis,the types of DSB was different;2.DSB in apoptosis is oligonucleosomal fragments and DSB in necrosis was non regular fragments;3.DSB occured more frequently or more densely in necrotic cells than that of in apoptotic cells.

关 键 词：细胞凋亡 细胞坏死 缺口翻译标记 DNA断裂 大鼠 

分 类 号：R329.26[医药卫生—人体解剖和组织胚胎学]