新生大鼠颅骨成骨性细胞体外生长过程研究  

BIOLOGICAL STUDY OF GROWTH PATTERN OF NEWBORN RAT CALVARIA OSTEOBLASTIC CELL IN VITRO

作　　者：刘祖德[1] 臧鸿声[1] 欧阳跃平[1] 

机构地区：[1]第二军医大学长征医院骨科及创伤外科研究所

出　　处：《解剖学报》1995年第2期157-160,共4页Acta Anatomica Sinica

摘　　要：为了确定鼠颅骨成骨性细胞在体外的发育分化过程是否与体内成骨细胞表型相似，我们用分次酶消化法分离新生大鼠颅骨成骨性细胞，进行形态学观察、碱性磷酸酶（Ａ１Ｐ）活性表现、细胞对甲状旁腺素（ＰＴＨ）的反应和形成钙化骨基质结节等研究。结果表明，新生大鼠颅骨细胞在体外培养过程中有两个明显的生长阶段，即增生期和分化成熟期。在增生期细胞数目逐渐增多，形态不规则，胞膜上的Ａ１Ｐ活性低，对ＰＴＨ反应逐渐增强；在分化成熟期细胞增殖缓慢，呈多边形，且聚集重叠成细胞结节，出现钙化骨基质，Ａ１Ｐ活性增强，而对ＰＴＨ的反应在第９ｄ达到高峰后却逐渐减弱。我们认为鼠颅骨成骨性细胞在体外随时间进程有着不同的生长模式，逐渐发育分化为成熟的成骨细胞。To determine if the development and differentiation of osteoblastic cell from rat calvaria in vitro is similar to that of osteoblast phenotype in vivo,osteoblastic cells were isolated by sequential enzyme digestion from newborn rat calvaria,The changes of their morphology,enzyme activity of alkaline phosphatase(AlP) and biological response to PTH,as well as mineralization of bone nodules were studied in culture,The results demonstrated that the cell population exhibited definitely twogrowth-stages:proliferation and maturation stages. In proliferation stage,the number of cells was increased,the morphology of cells was nonhomogeneous,the activity of AIP was weak and the response to PTH was increased.In maturation stage,the cell population grew slowly,exhibited polygonal shape,and became multilayer,clumped gradually and formed mineralization of bone nodules. The activity of AIP became stronger,the biological response to PTH reached a peak at 9th day and then declined.The authors suggested that osteoblastic cells from newborn rat calvaria presented the different biological patterns in vitro and gradually developed and differentiated into mature osteoblastic phenotype.

关 键 词：成骨性细胞 碱性磷酸酶 大鼠 颅骨 体外生长 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学]