联合固氮菌株分泌能力及其对燕麦的促生效应测定  

Porperty of associative nitrogen-fixing bacteriaproducing IAA and its promoting growth of oat

作　　者：席琳乔[1] 姚拓[1] 杨俊基 韩文星[1] 张德罡[1] 

机构地区：[1]甘肃农业大学草业学院,甘肃兰州730070 [2]甘肃省草原技术推广总站,甘肃兰州730070

出　　处：《草原与草坪》2005年第4期25-29,共5页Grassland and Turf

基　　金：甘肃省科学技术攻关项目(2GS035-A41-001-04);国际原子能机构项目(CPR-5-014);草业科学王栋奖学金资助

摘　　要：测定了分离自草坪草和燕麦根际的固氮菌株的分泌植物生长素(IAA)能力及其对燕麦的促生效应.结果表明:11株固氮菌都能分泌IAA,其中加前体(色氨酸)浓度在2.16～22.3μg/mL的有9株浓度大于10μg/mL;不加前体浓度在0.81～13.8 μg/mL的有6株浓度大于10μg/mL,有6株能够溶解有机磷,3株能够溶解无机磷,且溶磷强度在58～82,μg/mL,1株既能溶解有机磷,又能溶解无机磷;试管试验中除了2株效果比对照差外,其他9株株高增加62.8%～142.8%、叶绿素增加2.78%～63.89%、地上植物量增加21.45%～150.48%、地下植物量增加2.04%～70.75%、总根长增加2.27%～41.27%、根表面积增加11.32%～84.9%;另外I 017和I 012两个菌株分泌激素能力较好,I 016菌株综合能力较好.Associative nitrogen-fixing bacteria（ANFB） were isolated from turfgrass and forage （Oat） by using CCM and NFM medium . The ability of bacteria, which including fixing nitrogen, producing IAA, solubilizing phosphorus were determined. The results show that nine in eleven ANFB strains can produce auxin （IAA）, IAA concentration with L-Trp ranges 2. 16-22.3 μg/mL （9 strains over 10 μg/mL）, without L-Trd is 0.81- 13.8 μg/mL（6 strains over 10 μg/mL） ; 6 strains dissolve organic phosphorus,3 strains dissolve inorganic phosphorus （phosphate solubilizing power reaching 58-82 μg/mL）, one with both funtion; in experiment 9 strains show positive effect, shoot height increased 62.8 % - 142.8 %, which chlorophyll 2.78% - 63.89 %, A-bove ground dry weight 21.45% - 150.48%, Under ground dry weight 62.8% ~ 142.8% ,Total length of root 2.27%-41.27%, Root surface area 11.32%-84.9%; I 017 and I 012 were better in producing auxin, I 016 was better in comprehensive ability.

关 键 词：IAA 溶磷 联合固氮 草坪草 燕麦 

分 类 号：Q939.113[生物学—微生物学]