不同外植体对多年生黑麦草愈伤组织诱导的影响  

Effect of different explants on callus induction of perennial ryegrass

作　　者：陈季琴[1] 韩烈保[1] 杨纯奇[2] 李雪[1] 

机构地区：[1]北京林业大学草坪研究所,北京100083 [2]湖北鄂州大学成教处,湖北鄂州436000

出　　处：《草原与草坪》2005年第4期42-46,50,共6页Grassland and Turf

基　　金：国家转基因植物研究与产业化专项(J2002-B-006);863项目(2004AA244080)资助

摘　　要：对多年生黑麦草的3个品种进行愈伤组织诱导,选取成熟种子、成熟胚、胚轴、胚根4种外植体.结果表明,胚轴、胚根诱导效果较差,成熟胚在愈伤组织的诱导率、鲜重及愈伤质量方面均优于成熟种子.2,4-D浓度在2～4 mg/L,6-BA浓度为0.025 mg/L时有利于成熟胚诱导产生愈伤组织;成熟种子为外植体时,2,4-D浓度在5～8 mg/L,6-BA浓度在0.025 mg/L或0.2 mg/L有利于成熟种子诱导产生愈伤组织.2,4-D浓度为2 mg/L,6-BA浓度为0.5 mg/L时成熟胚和成熟种子诱导的愈伤组织鲜重均达到最大.特拉华在愈伤组织的诱导率、增殖速度及愈伤质量方面表现较优,托亚次之,尤文图斯较差.AC和LH有利于植株的再生.Three perennial ryegrass （Lolium perenne） varieties were cultured for callus induction on MS basic medium containing different concentration of 2,4-D and 6-BA by using different explants. The result showed that hypocotyls and radicels were ineffective in callus induction; 2-4 mg/L 2,4-D with 0.025 mg/L 6-BA were propitious to callus induction from mature embryos; 5-8 mg/L 2,4-D with 0. 025 mg/L or 0.2mg/L 6-BA were propitious to callus induction from mature seeds; the fresh weight of callus reaches the largest with 2 mg/L 2,4-D and 0.5 mg/L 6-BA of the three varieties. Delaware proved to be the best of the three, comes Taya and Juventus is the worst. AC and LH are effective in shoot regeneration.

关 键 词：多年生黑麦草 外植体 成熟种子 成熟胚 愈伤组织诱导 

分 类 号：S336[农业科学—作物遗传育种]