高效液相色谱法同时测定复方苯甲酸酊中两组分含量  

SIMULTANEOUS DETERMINATION OF THE CONTENTS OF BENZOIC ACID AND SALICYLIC ACID IN COMPOUND BENZOIC ACID TINCTURE BY HPLC

作　　者：王中兰[1] 冯碧敏[1] 张昊[1] 陈丽娟[1] 

机构地区：[1]泸州医学院附属医院药剂科,四川泸州646000

出　　处：《泸州医学院学报》2005年第4期360-362,共3页Journal of Luzhou Medical College

摘　　要：目的建立以高效液相色谱法测定复方苯甲酸酊中苯甲酸和水杨酸含量的方法。方法以TIANHEKromasilC18(5μ100A。4.6×250mm)为色谱柱,流动相为甲醇-水(60∶40),检测波长为274nm。结果苯甲酸平均加样回收率为100.1%,RSD为0.75%,水杨酸平均加样回收率为100.2%,RSD0.81%。结论本法简便、快速、准确,可用于复方苯甲酸酊的含量测定。Objective. To develop an HPLC method for simultaneous determination of benzoic acid and salicylic acid in compound benzoic acid tincture. Methods： Chromatography was conducted with TIANHE Kromasil C18 column （5μ 100A 1.6 × 250mm ）and mobile phase of mixture of methanol - water （60：40） ; the detection wavelength was 274nm. Rosults： The average recovery of benzoic acid was 100.1% and RSD was 0.75%. The average recovery of salicylic acid was 100.2% and RSD was 0.81%. Gonclusin： This method is simple, quick, and accurate, which can can used for the detemainafion of the tincture.

关 键 词：复方苯甲酸酊 苯甲酸 水杨酸 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]