Clinical Value of the Fluorescent Quantitative Polymerase Chain Reaction in the Diagnosis of Primary Syphilis  

荧光定量聚合酶链反应在一期梅毒诊断中的临床意义探讨(英文)

作　　者：朱慧兰 曾序春 叶兴东 武明昌 曾仁山[3] 

机构地区：[1]广州市皮肤性病防治研究所 [2]深圳市皮肤性病防治研究所 [3]广州市第一人民医院皮肤科

出　　处：《Chinese Journal of Sexually Transmitted Infections》2005年第1期21-23,共3页中华性传播感染杂志（英文版）

摘　　要：Objective： To evaluate the clinical value of fluorescent quantitative polymerase chain reaction （FQ-PCR） in the diagnosis of primary syphilis. Methods： 68 swab specimens were collected from patients suspected of infecttion with primary syphilis attending two STD clinics （Guangzhou Institute of Dermatovenerology and the First People＇s Hospital of Guangzhou city）, from September 1998 to December 2000. Analysis： by FQ-PCR, darkfield microscopy （D-F） for Treponema pallidum （TP）, and serologic testing for syphilis （STS）. Results： Of 68 patients, 30 （44.12%） were positive for TP by QF-PCR assay, 19 （27.94%） were positive for TP by D-F, 33 （48.53%） were positive for TP-IgG antibody by RPR, and 42 （61.76%） were positive for TP-IgG antibody by TPHA. There are significant differences in detection between D-F and TPHA （P〈0.05）, but there is no difference with RPR （P〉0.1）. Conclusion： This data shows that QF-PCR is a convenient, reliable and rapid method for diagnosis of primary syphilis, and may be an effective clinical assay in the detection of TP.目的为评价荧光定量聚合酶链反应(FQ-PCR)在一期梅毒诊断中的临床应用价值,以暗视野(D-F)、血清学(RPR/TPHA)检查方法作对照,用FQ-PCR方法检测68例疑诊为一期梅毒病人的生殖器溃疡处分泌物。在68例疑诊为一期梅毒病人中,D-F阳性率27.94%(19/68),RPR阳性率48.53%(33/68),TPHA阳性率61.76%(42/68),FQ-PCR阳性率44.12%(30/68),FQ-PCR与D-F、TPHA比较有显著性差异(P<0.05),与RPR比较无显著性差异(P>0.1)。本研究表明FQ-PCR检测生殖器溃疡TP在一期梅毒诊断中有一定的价值。

关 键 词：primary syphilis DIAGNOSIS fluorescentquantitative polymerase chain reaction 

分 类 号：R759.1[医药卫生—皮肤病学与性病学]