^(60)Co辐射高羊茅性状变异研究  

A Study on character mutation of^(60)Cor-ray irradiation inFestuca arundinacea

作　　者：张彦芹[1] 贾炜珑[2] 杨丽莉[1] 郭先龙[1] 吴锜[3] 胡鸢雷[3] 林忠平[3] 

机构地区：[1]山西省农业科学院旱地农业研究中心,山西太原030031 [2]天津大学理学院,天津300072 [3]北京大学生命科学学院,北京100871

出　　处：《草业学报》2005年第4期65-71,共7页Acta Prataculturae Sinica

基　　金：国家863项目--草类转基因研究(2001AA212161);院高新技术项目--RAPD技术在选择突变耐旱草坪草上的应用研究(YGX0501)。

摘　　要：本研究以高羊茅爱瑞3号为材料,用60Co-r射线照射干种子和分化苗,研究其适宜诱变剂量及性状变异。结果表明,出苗率和植株存活率随照射剂量增加而降低,干种子适宜诱变剂量为100~150Gy,分化苗适宜诱变剂量为20~25Gy;辐射当代材料在植株形态性状和耐旱(寒)性状上均发生了明显变异,其变异频率分别为1.52%~8.33%、1.52%~1.85%;60Co-r射线辐射可以获得叶片变小、变细且具有耐旱(寒)特性的高羊茅突变体;RAPD(随机PCR扩增)分析显示了这些突变体在DNA水平上发生的变异。Using the material of Festuca arundinacea Airei Ⅲ , suitable induced mutation doses and shape and property variation were studied by using 6^Co r-ray radiation to irradiate both dry seeds and differential seeding. The results showed that emergence and survival rates of individual plants decreased with increases in irradiation dosages. The appropriate induced mutation dosage was found to be from 100Gy to 150Gy for dry seeds and 20Gy to 25Gy for differential seeding. Radiation modern has an obvious variation in both plant shape and drought （cold） resistance character, spectively. 60^Co r-rays irradiation is more likely to obtain mutation with plants that have smaller and slimmer leaves with the character of drought （frigidity） resistance. Mutant variations were found at the DNA level through the use of RAPD analyzing.

关 键 词：高羊茅 辐射诱变 突变体 性状变异 

分 类 号：S54[农业科学—作物学] S688.4