真菌诱导型植物表达载体pBP的构建  

Construction of Fungi Inducible Plant Expression Vector pBP

作　　者：罗齐军[1] 曾富华[1] 吴永饶[2] 

机构地区：[1]湛江师范学院生化学院,广东湛江524048 [2]湖南农业大学生化与发酵工程实验室

出　　处：《湛江师范学院学报》2005年第3期37-40,共4页Journal of Zhanjiang Normal College

基　　金：广东省科技计划项目(Z002A2070402);湛江市科技攻关项目(103).

摘　　要：根据prp1-1启动子序列设计并合成了一对引物,通过PCR扩增的方法从马铃薯基因组中扩增到了病原诱导的prp1-1启动子,并将其克隆到PGEMT-Easy载体上,然后进一步克隆到植物表达载体pBI101.2上,构建了病原诱导型基因表达载体pBP.According to the reported sequences of inducible expression promoter prp1 - 1, a pair of primers were designed and synthesised. After the fragment of prp1 - 1 promoter was amplified by the method of PCR from the potato, it was inserted into PGEMT- Easy vector. Thereafter, the fragment was cloned into pBI101. 2 expression vector, and a new kind of pathogen - inducible expression vector pBP was constructed.

关 键 词：诱导型启动子 prp1-1 克隆 载体构建 

分 类 号：Q189[生物学—神经生物学]