阿维菌素B_(1a)组分高产菌株的诱变育种  

Mutagenesis and Screening of high Avermectin B_(1a) Producing Strains from Streptomyces Avermitilis

作　　者：任超[1] 马珦玻[2] 

机构地区：[1]天津现代职业技术学院,天津300222 [2]河北职工医学院,保定071000

出　　处：《生物技术通报》2005年第4期59-63,共5页Biotechnology Bulletin

摘　　要：目的了解阿维菌素产生菌S.avermitilis的生长特性,提高产生菌B1a组份的产量。方法采用紫外线(UV)、诱变剂氯化锂(LiCl),亚硝基胍(NTG)并结合甲硫氨酸(Met)诱导等手段对出发菌株S.avermitilisH0065进行诱变处理,初筛、复筛。结果筛选获得总效价达到4524.3μg/ml的高产阿维菌突变株N-3-133,其中B组分含量显著提高且高达85.3%,B1a也显著提高达到2533.6μg/ml。结论采用紫外线及亚硝基胍诱变方法,结合甲硫氨酸诱导筛选,与出发菌株相比可以获得阿维菌素总效价及B1a组分显著提高的菌株。Objective ：Study the chatacteristics of avermectin producing strains and improve avermectin B1a yield of Streptomyces avermitilis. Method：UV ,LiCl and NTG were used as mutagen in treating original strain H0065, including inducing by methionine. Results： The high-yield avermectin strain N-3-133 with avermectin total titer up to 4524.3 ug/ml was obtained,among them strain N-3-133 also was confirmed being a stable high-yield avermectin B producer which produced 85.3% avermectin B,also was avermectin B1. titer with up to 2533.6μg/ml. Conclusion： It is a effective way to obtain high-yield avermectin and B1a producer compared with the original strains by using UV and NTG as mutagen, Met as inducer.

关 键 词：阿维菌素 B1a组分高产菌株 诱变育种 甲硫氨酸 阿维链霉菌 杀螨剂 

分 类 号：Q933[生物学—微生物学]