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出 处:《第三军医大学学报》2005年第15期1599-1600,共2页Journal of Third Military Medical University
摘 要:目的观察新辅助化疗对宫颈鳞癌细胞增殖及凋亡的影响。方法对24例宫颈鳞癌进行1个疗程的以顺铂为主联合羟基喜树碱、博来霉素方案的新辅助化疗,以流式细胞术检测化疗前后的宫颈鳞癌组织中增殖细胞核抗原(PCNA)的表达;TUNEL法检测肿瘤细胞的凋亡指数(AI)。结果化疗临床有效率为58.3%;化疗后宫颈鳞癌组织中PCNA的表达由(0.386±0.078)降至(0.125±0.040),差异显著(t=22.859,P<0.001),而AI由(0.052±0.027)升至(0.248±0.078),差异显著(t=16.06,P<0.001);化疗后临床有效者AI升高程度高于无效者,差异显著(t=2.398,P=0.029)。结论对宫颈鳞癌进行顺铂联合羟基喜树碱、博来霉素的新辅助化疗能显著抑制肿瘤细胞增殖、增加凋亡,PC-NA表达的下调及AI上调可能是新辅助化疗抗癌作用的重要机制之一。Objective To determine the effect of neoadjuvant chemotherapy (NAC) for cervix cancer on cell proliferation and apoptosis. Methods A total of 24 patients with squamous carcinoma of the cervix (SCC) were treated with one cycle of cisplatin combined with hydroxycamptothecine, bleomycin regimen. The expression of proliferating cell nuclear antigen (PCNA) and apoptosis index (AI) were detected before and after NAC with flow cytometry and TUNEL. Results Clinical response was 58.3 % (14/24). Before and after NAC, the expression of PCNA were (0. 386 ±0. 078) and (0. 125±0. 040) respectively and AI were (0. 052±0. 027) and (0. 248±0. 078 ). Significant difference of PCNA and AI could be observed before and after NAC (t = 22. 859, t = 16. 06, P 〈 0.01 ). The mean AI after NAC was significantly higher in effective cases (t = 2. 398, P = 0. 029). Conclusion NAC with cisplatin, hydroxycamptothecine, bleomycin regimen could inhibit proliferation and increase apoptosis of tumor cell in cervical squamous carcinoma tissue. The change of PCNA expression and AI may be one of molecule mechanisms leading to anti-tumor function of NAC.
分 类 号:R329.28[医药卫生—人体解剖和组织胚胎学] R730.53[医药卫生—基础医学]
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