急性低氧和间断低氧习服对HepG2细胞内血管内皮细胞生长因子基因表达的影响  

EFFECTS OF ACUTE HYPOXIA AND INTERMITTENT HYPOXIC ACCLIMATIZATION ON VASCULAR ENDOTHELIAL GROWTH FACTOR GENE EXPRESSION IN HepG2 CELLS

作　　者：陈伟[1] 陈家佩[2] 葛世丽[2] 付小兵[1] 从玉文[2] 

机构地区：[1]解放军第304医院全军创伤修复重点实验室,北京100037 [2]军事医学科学院放射医学研究所,北京100850

出　　处：《中国应用生理学杂志》2005年第3期274-277,共4页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金重点课题(39730190);国家重点基础研究规划资助项目(G1999054204)。

摘　　要：目的:观察急性低氧和间断低氧习服对人HepG2细胞内血管内皮细胞生长因子(VEGF)及转录因子低氧诱导因子-1α(HIF-1α)的mRNA和蛋白含量的影响及其可能的生物学意义。方法:HepG2细胞随机分为常氧对照组,急性低氧组和间断低氧习服组。采用Northernblot和Westernblot分别检测不同组别HepG2细胞内VEGF和HIF-1αmRNA表达和蛋白含量的变化。结果:急性低氧诱导HepG2细胞内VEGF和HIF-1α基因的转录,增加两种蛋白在细胞内的含量。间断低氧习服组的细胞内VEGF和HIF-1α的mRNA含量分别为常氧对照组细胞的(108.6±17.7)%和(116.74±19.8)%,与常氧对照组相比无显著差异(P>0.05);而其蛋白表达的含量分别为对照组细胞的1.4和2.7倍,都明显低于急性低氧组细胞内两种蛋白的含量(P<0.05)。结论:HepG2细胞达到低氧习服状态后,抑制急性低氧对HepG2细胞内VEGF基因表达的促进作用,其中HIF-1α可能起着重要的调节作用。Aim： To explore the influence of acute hypoxia and intermittent hypoxic acclimatization on vascular endothelial growth factor （VEGF） and hypoxia-inducible factor 1α（HIF-1α） gene expression in HepG2 cells underlying their possible biological significance. Methods： HepG2 was cultured in 1% O2 for 24 hours, then in 21% O2 for another 24 hours, which composed a hypoxic exposure cycle. After 6 cycles, HepG2 cells reached the status of hypoxic acclimatization. Gene transcription and translation of VEGF and HIF-1α were detected with Northern blot and Western blot methods. Results： Acute hypoxia could induce gene transcription and translation of VEGF and HIF-1α. After intermittent hypoxia acclimatization, the contents of VEGF and HIF-1α mRNA were 108.6% ± 17.7 % and 116.7 % ± 19.8 % of those in normoxic control cells, while the protein contents were significantly increased to 1.4 and 2.7 times of those in control cells, respectively（ P 〈 0.05）. The protein expression levels of VEGF and HI F-1α were decreased in cells subjected to hypoxia acclimatization compared to cells treated with acute hypoxia. Conclusion： When HepG2 cells reached the status of hypoxic acclimatization, the acute hypoxia-induced increment of VEGF gene transcription and translation in cells were inhibited, in which HIF-1α might play an important role.

关 键 词：急性低氧 间断低氧习服 血管内皮细胞生长因子 低氧诱导因子-1Α 

分 类 号：Q151.93[生物学—普通生物学] R852.11[医药卫生—航空、航天与航海医学]