核因子-κB在大鼠慢性阻塞性肺疾病肺组织中的定位及表达  

LOCATION AND EXPRESSION OF NF-κB IN LUNG OF RATS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE

作　　者：林书典 戴爱国 席守民[2] 

机构地区：[1]湖南省老年医院,湖南省老年医学研究所呼吸疾病研究室,湖南长沙410001 [2]河南科技大学医学院生物化学教研室,河南洛阳471003

出　　处：《中国应用生理学杂志》2005年第3期293-295,i0003,共4页Chinese Journal of Applied Physiology

基　　金：湖南省科技厅科研基金资助项目(01JZY2017)

摘　　要：目的:研究转录调控因子———核因子-κB(NF-κB)在大鼠慢性阻塞性肺疾病(COPD)模型肺组织中的定位和表达,探讨NF-κB在COPD发病中的作用与意义。方法:雄性Wistar大鼠14只,随机分为COPD模型组和对照组,每组7只。采用每日熏香烟和两次气管内滴入200μg脂多糖法制作COPD大鼠模型。用免疫组化和原位杂交法检测肺中NF-κBp65蛋白表达及其mRNA表达。结果:免疫组化发现,COPD模型组大鼠肺NF-κBp65高表达于肺泡、支气管上皮细胞和小动脉内皮细胞胞核中(其光密度半定量分析分别为0.426±0.007,0.434±0.012和0.313±0.007,P均<0.01),而对照组在相应部位仅有少量表达或不表达(分别为0.115±0.006,0.116±0.005和0.095±0.007)。原位杂交显示,NF-κBp65在COPD组高表达于肺泡上皮细胞,支气管、小动脉平滑肌细胞胞浆中(分别为0.203+0.008,0.208±0.010和0.206±0.007P均<0.01),而对照组呈低表达(0.100±0.006,0.102±0.002和0.103±0.003)。结论:核因子-κB在慢性阻塞性肺疾病中的表达、激活和核转位可能是众多炎性基因表达的基础,广泛分布于多种细胞中,参与对其基因的转录调控。Aim： To elucidate the location and effects of transcription factor-nuclear factor-κB（NF-κB） in lung tissues of rats with chronic obstructive pulmonary disease（COPD）. Methods： Fourteen male Wistar rats were randomly divided into COPD model and control groups equally. The COPD model was established by intratracheal instillation of lipopolysaccharide twice and exposure to cigarette smoke daily. We detected the NF-κB p65 protein in lung by immunohistochemic method, and the expression of NF-κB p65 mRNA in lung by in situ hybridization. Results： Immmunohistochemistry, the expression of NF-κB p65 protein in alveolar, bronchiolar epithelium and arteriolar endothelium was significantly higher in the COPD group（0. 426 ± 0. 007, 0. 434 ± 0. 012 and 0. 313 ± 0. 007, respectively） than those of the control group （0.115 ± 0. 006, 0.116 ± 0. 005 and 0. 095 ± 0. 007, respectively, all P 〈 0.01 ）. In situ hybridization showed that the expressions of NF-κB p65 mRNA in alveolar epithelium（0. 203 ± 0. 008）, bronchiolar and arteriolar smooth muscle cell（0.208 ± 0. 010 and 0. 206 ± 0. 007） of rats in the COPD group were stronger than those in the control group （0. 100 ± 0. 006, 0. 102 ± 0. 002 and 0. 103 ± 0. 003 respectively） by semiquantitative analysis（all P〈 0.01 ）. Conclusion： The expression and nuclear translocation of NF-κB may be the basis event of gene expression of many cytokines and inflammatory mediators, which may positively regulate gene expression of many cytokines and inflammatory mediators in various cell lines.

关 键 词：核因子-κB(NF—κB) 慢性阻塞性肺疾病 调控 

分 类 号：Q74[生物学—分子生物学] R45[医药卫生—治疗学]