高糖高胰岛素对去甲肾上腺素诱导的心肌细胞肥大的影响  

EFFECT OF HIGH GLUCOSE AND HIGH INSULIN ON NE-INDUCED CARDIAC HYPERTROPHY OF THE CULTURED MYOCARDIAL CELLS

作　　者：周庆峰[1] 王洪新[1] 王怡薇[1] 符丽娟[1] 单丹[1] 刘鹤[1] 

机构地区：[1]锦州医学院药理学教研室,辽宁锦州121001

出　　处：《中国应用生理学杂志》2005年第3期305-309,共5页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助课题(39870356)

摘　　要：目的:利用体外培养的乳鼠心肌细胞,观测高浓度胰岛素和高浓度葡萄糖作用下对去甲肾上腺素诱导的心肌细胞肥大的影响,并探讨其可能作用机制。方法:以培养的乳鼠心肌细胞为模型分组给药后,用显微镜目镜计数心肌细胞搏动的频率;用Lowrys法测心肌细胞的蛋白质含量;用消化分离法,利用计算机图象分析系统测心肌细胞的体积;用[3H]leucine标记法测定心肌细胞蛋白的合成。结果:与对照组相比,去甲肾上腺素(NE)组、高糖组、高胰岛素组心肌细胞蛋白含量、体积、蛋白合成均有明显增加,而与高糖加NE组和高糖高胰岛素组相比较,高糖高胰岛素加NE组心肌细胞蛋白含量、体积、蛋白合成增加则更为显著。结论:单纯高浓度胰岛素培养可促进心肌细胞肥大,同时用高糖高胰岛素联合培养,可使去甲肾上腺素诱导的心肌细胞肥大作用进一步增强。Aim： To study the effect of diabetes-like environment on the cardiac hypertrophy, cultured cardiomyocytes were used to study the effect of high insulin and high glucose on norepinephrine（NE）-induced cardiac hypertrophy. Methods： Using cultured myocardial cells as a model, the cellular hypertrophy was observed. The contracting frequency was counted by the inverted microscope, the protein content was assayed with Lowrys method, the cardiomyocytes＇ volumes were measured by computer photograph analysis system, the protein synthesis was assayed with [^3H] leucine intake method. Results： The total cellular protein content, cellular volumes, cellular protein synthesis showed an increase in high insulin group and high glucose group compared with control group. High insulin and high glucose and NE group showed a further increase compared with high glucose and NE group. Conclusion： The high insulin itself induces hypertrophy of the cultured myocardial cells slightly. Meanwhile, imitating diabetes-like environment with high insulin and high glucose and NE can further accelerate hypertrophy of the cultured myocardial cells.

关 键 词：高糖 高胰岛素 肥大 胰岛素抵抗 

分 类 号：Q487[生物学—生理学] Q463