肝灵对大鼠胆汁淤积性肝硬化的抗肝纤维化作用  

The anti-hepatic fibrosis effects of Ganling

作　　者：胡秀萍[1] 吴樱樱[1] 朱虹[1] 徐明[1] 邹宇宏[1] 邝荔香[1] 杨雁[1] 陈敏珠[1] 

机构地区：[1]安徽医科大学药理教研室,合肥230032

出　　处：《安徽医科大学学报》2005年第4期316-318,共3页Acta Universitatis Medicinalis Anhui

摘　　要：目的探讨肝灵对大鼠胆汁淤积性肝硬化的抗肝纤维化作用。方法利用胆总管结扎术(bile duct ligation,BDL)制备大鼠胆汁淤积性肝硬化模型,于造模第4周起ig肝灵8g/(kg·d),检测相关的血液生化指标、血液流变学、肝匀浆中SOD活性、丙二醛(MDA)和羟脯氨酸(HyP)的含量以及肝脏病理组织学的改变。结果肝灵不仅能明显降低肝、脾指数、血浆ALT以及肝组织中的HyP含量,同时使肝硬化大鼠的肝组织SOD活性和总蛋白(TP)明显升高,而且明显降低四种不同切变率(1/200s、1/30s、1/5s、1/s)的全血黏度值、全血高切和低切相对黏度以及血浆黏度值,显著减少肝硬化大鼠的腹水量以及明显增加24h尿钠、钾的排泄量,病理组织学结果显示,可使间质炎细胞浸润减少,能减轻胆汁淤积性肝硬化大鼠的肝细胞损伤和肝纤维化程度。结论肝灵对胆总管结扎所致大鼠胆汁淤积性肝硬化及其并发症腹水有明显的治疗作用,可能与其抗炎、抗氧化、活血化淤、排钠排钾等机制有关。Objective To explore the effect of Ganling on hepatie fibrosis of cholestasis and its mechanisms.Methods The rat model of hepatic fibrosis of cholestasis was induced by common bile duct ligation,and the samples of liver and blood were measured.The damage of liver tissues was observed by light microscope.Results Compared with the modle group,there were five changes in Ganling treated group.①Liver index,spleen index,ALT in blood and hydroxyproline level of liver were significantly reduced in Ganling treated group.②SOD activity and total protein of liver were obviously improved.③Ganling significantly depressed seven kinds of conglutinations of the blood.④Ganling significantly reduced aseites and inereased the exeretion of natrium,kalium in 24h urine.⑤Pathology results showed that Ganling significantly amended liver cirrhosis,lightened the trauma of liver cell,reduced the infiltration of inflammation cell.Conclusion Ganling effectively suppresses hepatic fibrosis and ascites by these mechanisms inclading anti-inflammatory,anti-oxidation,exeretion-increaseing of natrium and kalium,blood-activating and stasis-aliminating.

关 键 词：肝硬化 胆汁性/药物疗法 胆总管/外科学 

分 类 号：R977.1[医药卫生—药品] R575.2[医药卫生—药学]