黑眶蟾蜍和黑斑蛙消化道5-羟色胺免疫活性细胞的免疫组织化学  

Immunohistochemical Study on 5-Hydroxytryptamine Immunoreactive Cells in the Alimentary Tract of Bufo melanostictus and Rana nigromaculata

作　　者：张秋金[1] 陈友铃[1] 耿宝荣[1] 王义权[2] 

机构地区：[1]福建师范大学生物工程学院,福州350007 [2]厦门大学生命科学学院,厦门361005

出　　处：《动物学杂志》2005年第4期17-21,共5页Chinese Journal of Zoology

基　　金：福建省自然科学基金(No.D0320001)

摘　　要：对黑眶蟾蜍(Bufomelanostictus)和黑斑蛙(Rananigromaculata)消化道5-羟色胺免疫活性细胞的形态、分布进行了免疫组织化学定位。5-羟色胺免疫活性细胞在黑眶蟾蜍和黑斑蛙消化道各段中均有分布,分布密度均呈升-降-升-降的波浪式分布特点,二者在幽门部和回肠都有个分布的高峰值。黑眶蟾蜍回肠最高,空肠、幽门部次之,十二指肠、直肠最低;黑斑蛙幽门部最高,回肠、空肠次之,食道、贲门部、直肠最低。5-羟色胺免疫活性细胞位于胃的胃腺上皮、食道及肠的粘膜上皮,有圆形、椭圆形、梭形、楔形等,有的有胞突。文中讨论了5-羟色胺免疫活性细胞分布型的原因及形态与功能的关系。Using immunohistochemical technique, 5-Hydroxytryptamine immunoreactive （5-HT-IR） cells were observed in the alimentary tract of the toad Bufo rnelanostictus and the frog Rana nigromaculata. 5-HT-IR cells were distributed throughout the alimentary tracts of both species and located in the gastric glands of the stomach regions and in the intestinal or esophageal epithelium with variable frequencies. The density of the cell in B. melanostictus is the highest in the ileum, moderate in the jejunum and stomachus pyloricus, and the lowest in the duodenum and rectum. In R. nigromaculata the density of 5-HT-IR cells is the highest in the stomachus pyloricus, moderate in the ileum and jejunum, and the lowest in the esophagus, stomachus cardiacus and rectum. 5-HT-IR cells were spherical, elliptoid, coniform or spindle-shaped with long cytoplasmic process reaching to the lumen. The distribution mode of 5-HT-IR cells and the relationship between morphology and function are also discussed.

关 键 词：黑眶蟾蜍 黑斑蛙 消化道 5-羟色胺免疫活性细胞 免疫组织化学 

分 类 号：Q593[生物学—生物化学]