烟粉虱内共生菌groEL基因的原核表达条件研究  

Studies on the Prokaryotic Expression Conditions of groEL from Endosymbionts in Bemisia tabaci

作　　者：谭周进[1] 谢丙炎[2] 肖启明[3] 杨宇红[2] 冯兰香[2] 

机构地区：[1]湖南农业大学食品科技学院,长沙410128 [2]中国农业科学院蔬菜花卉研究所,北京100081 [3]湖南农业大学生物安全科技学院,长沙410128

出　　处：《微生物学通报》2005年第4期57-61,共5页Microbiology China

基　　金：国家重点基础研究发展规划项目(No.2002CB111400)

摘　　要：为了获得较多的GroEL蛋白,深入研究其性质,对烟粉虱内共生菌groEL基因表达的条件进行了研究.结果表明:诱导groEL基因原核表达的最佳IPTG浓度为100μmol/L;在35℃诱导培养,能够获得较高的蛋白表达量;最佳诱导培养时间为4～5h;最佳诱导培养的初始pH值为8.0;在振荡转速为120r/min、诱导培养时间为4～5h时,增大接种量,有利于原核基因的表达;添加少量的NH4+有利于提高groEL基因原核表达的量,但Ca2+、Fe3+、K+、Mg2+则抑制groEL基因的原核表达,其中Fe3+抑制作用最为强烈;NH4+含量过高也不利于groEL基因原核表达;在培养基中添加少量的葡萄糖,能够提高groEL基因原核表达,但葡萄糖含量较高时不利于groEL基因原核表达.The study on prokaryotic expression conditions of groEL from endosymbiont in B. tabaci was conducted. The results showed that： The optimal IPTG content for inducing prokaryotic expression of groEL was 100μmol/L When it was induced by IPTG at 35℃, higher GroEL product would be acquired. The optimal induced culturing time for prokaryotic expression of groEL was 4 - 5 hours. Higher inoculated quantity would benefit prokaryotic expression of groEL. A little NH4＋ content would improved prokaryotic expression of groEL, but prokaryotic expression of groEL would be inhibit by Ca^2 ＋ , Fe^3 ＋ , K ^＋ and Mg^2＋ . When low content glucose was added to the LB medium, it would benefit prokaryotic expression of groEL. Prokaryotic expression of groEL would be inhibit by high content glucose.

关 键 词：烟粉虱 内共生菌 GROEL 原核表达 理化因素 

分 类 号：Q786[生物学—分子生物学]