黄绿木霉原生质体诱变育种研究  

Protorlast Induced Mutation Breeding on Trichoderma aureoviride

作　　者：杨谦[1] 孙冬梅[1] 张晶[1] 

机构地区：[1]哈尔滨工业大学生命科学系,哈尔滨150001

出　　处：《微生物学通报》2005年第4期62-67,共6页Microbiology China

摘　　要：利用正交实验方法研究了影响黄绿木霉(Trichoderma aureoviride)原生质体形成的因素,并利用紫外线、硫酸二乙酯、氯化锂几种因素复合诱变原生质体筛选高产纤维素酶菌株。影响原生质体形成的因子顺序是酶系统>菌龄>酶解时间>酶解温度,原生质体形成的最佳条件是0.5%蜗牛酶+0.5%溶菌酶+1.0%纤维素酶,菌龄为18h,酶解时间为3.0h,酶解温度为32℃;在该条件下原生质体产量可达到4.25×106个mL-1。Ca2+和PEG对提高原生质体再生率的作用明显;复合诱变后得到酶活显著提高、遗传性能稳定的诱变株T-14,其EG酶活与BG酶活分别提高了58.43%、44.48%。Based of the study on the impact of protoplasts under different conditions of enzymolysis solution, culture time, enzymnlysis time and enzymolysis temperature in sequence using orthogonal test. The best optimal condition of producing protoplast was ： 0. 5% heliease add 0. 5% lysozyme and 1.0% cellulase, enzymolysis time was 3 hours , enzymolysis temperature was 32℃, under these condition the production of protoplast can be 4. 25 × 10^6per ml- Ca^2 ＋ and PEG can increase the regeneration ratio of protoplast. After induced mutation by complex factor under DES, UV and LiCl, the mutagenesis strain which own increasing enzyme activity and transmissibility steaty was obtained.

关 键 词：黄绿木霉 原生质体 诱变 育种 

分 类 号：Q933[生物学—微生物学]