螺旋藻乙醇酸氧化酶(GO)的研究  

Study on Glycolic Acid Oxidase (GO) of Spirulina(Arthrospira)

作　　者：王志忠[1] 巩东辉[1] 刘华[1] 乔辰[1] 

机构地区：[1]内蒙古农业大学农学院,呼和浩特010019

出　　处：《微生物学通报》2005年第4期68-71,共4页Microbiology China

基　　金：国家自然科学基金项目(No.30460104);内蒙古自然科学基金项目(No.990303-4)

摘　　要：采用比色法对鄂尔多斯高原碱湖的钝顶螺旋藻(S1)与国外引进的钝顶螺旋藻(S2)和极大螺旋藻(S3)的乙醇酸氧化酶(GO)进行了比较研究.结果表明:在25℃、pH 8.0条件下,S1、S2和S3的GO活性分别为70.9 U/gFW、59.6 U/gFW和80.9 U/gFW;最适温度均为30℃;在0℃～35℃(30℃)范围内比较稳定;最适pH值分别为8.6、8.2和8.4;pH值稳定范围,S1为7.6～10.0、S2为8.0～9.0;S3为8.0～8.6.S1的GO对温度和pH适应范围最宽,且在低温、高温、强酸和强碱下的活性均比引进种的高.A comparative study on the glycolic acid oxidase （GO） of the domestic Spirulina （Arthrospira） platensis （S1 ） from alkaline lake in Erdos Plateau and the imported S. （A.） platensis （S2） and S. （A.） maxima （ S3 ） as well is made with calorimetric method. The results show that activity of GO （25℃, pH 8. 0） of S1 , S2 and S3 is 70. 9 U/gFW, 59.6 U/gFW and 80. 9 U/gFW respectively; the GO＇s optimum temperature of S1 , S2 and S3is 30℃ ; the GO＇s optimum pH value of S1 is 8.6, while that of S2 8.2 and that of S3 8.4 ; the GO of S1 is stable from 0℃ to 35℃ and from pH 7.6 to pH 10. 0, while that of S2 from 0℃ to 30℃ and from pH 8.0 to pH 9. 0 and that of S3 from 0℃ to 35℃ and from pH 8.0 to pH 8.6. Adaptive range of S1 GO for temporature and pH is wider, and activity at low and high temperature and under strong acidand alkali conditions is higher than that of the imported species.

关 键 词：螺旋藻 乙醇酸氧化酶 酶动力学 鄂尔多斯高原碱湖 

分 类 号：Q946.5[生物学—植物学]