桔小实蝇分子生物学研究中样品采集及保存处理的改进方法  

An improving method about samples collecting and specimens preserving for Bactrocera dorsalis molecular biology study

作　　者：施伟[1] 叶辉[1] 

机构地区：[1]云南大学生命科学学院生物系,昆明650091

出　　处：《昆虫知识》2005年第4期460-462,共3页Entomological Knowledge

基　　金：国家重大基础研究计划"973"项目(2003CB415100);国家自然科学基金项目(30260023)。

摘　　要：介绍了一种果实蝇,桔小实蝇Bactroceradorsalis的虫样采集和保存处理的方法。利用一种简易的诱捕装置对于野外短时间、大面积采集实蝇虫样效果显著;同时,用TE溶液浸泡的干燥虫样适用于桔小实蝇的DNA抽提和PCR扩增。这种虫样采集和保存方法也适用于果实蝇属其它几类实蝇的分子生物学研究。A sample trapping device was used for samples collecting of Bactrocera dorsalis, a fruit fly of Bactrocera. This trapping device is suitable for large-scale sampling in field. The dry specimens of B. dorsalis preserved by TE solution was also suitable for DNA extraction and PCR amplification. The same samples collecting and specimens preserving methods were also fitted for molecular study in other several fruit flies of the genus Bactrocera.

关 键 词：桔小实蝇 采集 保存处理 分子生物学研究 样品采集 保存方法 改进方法 PCR扩增 DNA抽提 效果显著 

分 类 号：S433[农业科学—农业昆虫与害虫防治]