鞘内注射U0126对吗啡戒断大鼠脊髓神经元Fos表达的影响  

Effects of intrathecal injection of MEK inhibitors U0126 on the expression of Fos in spinal cord of morphine-induced withdrawal rats

作　　者：刘海林[1] 曹君利 戴培静[1] 郑国龙[1] 李广明[1] 

机构地区：[1]南京医科大学附属淮安市第一人民医院麻醉科,淮安223300 [2]江苏省麻醉医学重点实验室

出　　处：《南通大学学报（医学版）》2005年第4期235-237,共3页Journal of Nantong University(Medical sciences)

基　　金：国家自然科学基金资助项目(No:30200267);江苏省教委自然科学基金资助项目(01KJD320036)。

摘　　要：目的:研究鞘内注射U0126对吗啡依赖大鼠戒断反应中Fos表达的影响。方法:采用大鼠吗啡依赖及戒断模型,分为正常对照组、吗啡依赖组、吗啡戒断组、U0126组、DMSO组,运用免疫组织化学法(n=6)和Westernblot(n=4)方法观察鞘内注射U0126对吗啡戒断大鼠脊髓神经元Fos表达的影响。结果:鞘内注射U0126可明显减少L5节段脊髓背角Fos阳性神经元的数目,U0126组为287±54,低于戒断组380±71,两者差异有显著性(P<0.05)。Western blot显示U0126组Fos蛋白的表达与戒断组相比也明显减少。结论:鞘内注射U0126能明显抑制吗啡戒断大鼠脊髓神经元Fos表达。Objective： This study was undertaken to explore the effects of intrathecal injection of MEK inhibitors U0126 on the expression of Fos in spinal cord of morphine--induced withdrawal rats. Methods：All the rats were divided into 5 groups：a control group,a dependent group,a withdrawal group,a U0126 group （5μg）,and a DMSO group. Immunohistochemical and Western--blotting techiniques were undertaken to evaluate the expression of Fos in spinal cord respectively. Results： The results showed that the level of Fos-like positive neurons in dorsal horn of withdrawal group was 380±71, which was higher that of U0126 group,which was 287±54（P〈0.05）. Compared with the withdrawal group,the level of Fos protein in spinal cord of U0126 group was significantly lower. Conclusions ： The results indicates that MEK inhibitors U0126 can suppress the expression of Fos in spinal cord.

关 键 词：吗啡 戒断综合征 脊髓 U0126 细胞外信号调节激酶 大鼠 

分 类 号：R996[医药卫生—毒理学]