弱精子症患者精核蛋白含量的分析  

Analysis of Protamine Content in Patients with Asthenozoospermia

作　　者：陈松[1] 曹坚[1] 费仁仁[2] 毛全宗[1] 李汉忠[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院泌尿外科,北京100730 [2]中国医学科学院药物研究所,北京100050

出　　处：《中华男科学杂志》2005年第8期587-589,593,共4页National Journal of Andrology

摘　　要：目的:研究精核蛋白在男性不育诊治中的意义.方法:对199例临床确诊的弱精子症患者的精液提取精子总碱性核蛋白(简称精核蛋白,TNBP),进行电泳及扫描分析,并用总组蛋白(TH)与总精核蛋白(TP)的比值来表示组蛋白-精核蛋白的取代(HPRR)程度.结果:在199例患者中,42例(21.1%)TNBP含量正常,157例(78.9%)异常,其中99例(49.7%)TH/TP高于正常,属HPRR受阻;45例(22.6%)为HP1/(HP2+HP3)比值升高,属P2型精核蛋白减少;51例(25.6%)HP2+HP3全部缺失;44例(22.1%)TP全部缺失.从TNBP含量异常的157例患者中随机抽出30例进行2～7个月的有效治疗后,再次进行TNBP电泳及扫描分析,发现14例(46.6%)TNBP含量无明显变化;11例(36.6%)显著变化;5例(16.6%)TNBP含量恢复正常.结论:HPRR受阻和P2型精核蛋白异常与男性不育相关,TNBP含量的变化可作为不育患者治疗效果的监测指标之一.Objective： To study the significance of the change of protamine content in the management of male infertility. Methods ： Sperm nuclear proteins extracted from 199 infertile patients were analyzed by polyacryamide gel-electrophoresis and scanning microdensitometry. Results： Forty-two （21% ） cases of the total number had normal sperm nuclear proteins and 157 （79%） had aberrant ones, which mainly presented the interruption of HPRR and abnormality of P2 protamine. Thirty patients were selected at random from 157 abnormal cases for clinical treatment. After the treatment, the sperm nuclear proteins were extracted and analyzed and the results demonstrated that I l cases （36.6%） improved markedly, 5 （16.6%） restored to normal and the other 16 （46.6%） remained unchanged. Conclusion ： There is a reduced level or selective absence or even complete selective absence of protamines in infertile patients. Protamines may act as a parameter for evaluating the treatment effect of infertile males and protamine content can be influenced by a certain or several factors.

关 键 词：男性不育 弱精子症 精核蛋白 组蛋白-精核蛋白取代反应 

分 类 号：R698.2[医药卫生—泌尿科学]