非梗阻性无精子症患者不同部位睾丸组织鱼精蛋白-2 mRNA的测定  

Determination of Expression of Protamine-2 mRNA in Different Positions of the Testis of Patients with Nonobstructive Azoospermia

作　　者：邱学德[1] 杨宇如[1] 李响[1] 李永刚[2] 李春华[2] 

机构地区：[1]四川大学华西医院泌尿外科,四川成都610041 [2]云南省第一人民医院生殖遗传科,云南昆明650101

出　　处：《中华男科学杂志》2005年第8期590-593,共4页National Journal of Andrology

摘　　要：目的:探讨非梗阻性无精子症睾丸组织鱼精蛋白-2(P-2)mRNA的表达量与相应睾丸组织取精结果的关系.方法:无精子症38例,平均年龄32.4(24～42)岁,依据病理诊断结果分为非梗阻性无精子症组和梗阻性无精子症组.活检时每侧睾丸取上下两个不同部位的组织,并将其分为3部分,分别用作普通病理活检、精子检测和mRNA提取.用RT-PCR和图像分析系统测定P-2 mRNA的表达量.结果:38例患者中27例为非梗阻性无精子症,11例为梗阻性无精子症.非梗阻性无精子症组检出和未检出精子的部位在睾丸的分布无一定规律可循.检出精子和未检出精子的组织P-2 mRNA的表达量分别为1.40±0.21和0.51±0.23(P＜0.05).结论:非梗阻性无精子症患者取少量的睾丸组织测定P-2 mRNA的表达量可提示该部位睾丸组织精子分离的结果.Objective ： To investigate the relationship between the expression of protamine-2 （ P-2 ） mRNA and the results of sperm extraction in the corresponding testis tissues of patients with nonobstructive azoospermia. Methods ： Based on pathological diagnosis, 38 eases of azoospermia at the mean age of 32.4 （ ranging 24 ～ 42 ） years were divided into a nonobstructive （NOA） group and an obstructive （OA） group. Two specimens were taken from different positions of one testis, each divided into three portions for general pathological test, sperm separation and mRNA extraction, respectively. The expression of P-2 mRNA was determined by RT-PCR and image analysis assay. Results： Among the 38 cases, 27 were diagnosed as nonobstructive and 11 as obstructive azoospermia. No regularity was found as to the positions where sperm could or could not be successfully isolated. The expression of P-2 mRNA was 1.40 ± 0.21 in the tissues where sperm was isolated and 0.51 ± 0.23 （ P 〈 0.05 ） in those where no sperm was isolated. Conclusion ： The expression of P-2 mRNA in the testicular tissues from the patient with nonobstructive azoospermia could reveal the results of sperm extraction in the corresponding tissues.

关 键 词：无精子症 睾丸活检 精子分离 鱼精蛋白-2 MRNA 

分 类 号：R698.2[医药卫生—泌尿科学]