利用炮制技术去除关木通毒性成分的方法学研究  

Methodological studies on selectively removing toxins in Aristolochiae manshuriensis by Chinese processing techniques

作　　者：王智民[1] 由丽双[1] 姜旭[1] 李琳[1] 王维皓[1] 王冠[1] 

机构地区：[1]中国中医研究院中药研究所,北京100700

出　　处：《中国中药杂志》2005年第16期1243-1246,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金资助项目(30271612)

摘　　要：目的:根据马兜铃酸的化学性质,借鉴传统中药炮制经验,对去除马兜铃酸的炮制方法学进行研究。方法:以关木通为研究对象,马兜铃酸A为指标,采用正交试验等方法对关木通的碱制和醋制工艺进行优化。结果:确定了关木通的炮制工艺为以0.1mol·L-1碳酸氢钠多次浸泡后醋制的方法。炮制过程中温度对毒性成分的去除率影响较大。不同含量的关木通在实验室小试炮制中,毒性成分去除率在90%以上,10批中试毒性成分的去除率在80%以上,使马兜铃酸A的含量降低到0.35-0.60mg·g-1。马兜铃酸在药材中部分是盐的形式存在的。结论:该炮制新工艺能够选择性的去除关木通中的绝大部分毒性成分,达到了去毒的目的。Objective： To investigate the possibility of selectively detoxifying aristolochic acids in Aristolochiae manshuriensis （Guanmutong） by the chemical properties of aristolochic acids and traditional Chinese processing experience. Method： The technical parameters in processing technique of A.manshuriensis were optimized by orthogonal designed methods with aristolochic acid A. Result： The processing technique was soaked in 0.1 mol·L^-1 baking soda for several times and then processing with vinegar. Temperature was important factor to detoxify aristolochic acids, aristolochic acid A were removed over 90% from A.manshuriensis in laboratory and over 80% in medium-scale production by 10 batches from two origins of botanical drugs with different contents, and decreased to 0.35-0.60mg·g^-1 in processed products. aristolochic acid A existed mostly salt-forms in the botanical drug. Conclusion： Most toxic components in Guanmutong could be removed by the new processing method.

关 键 词：马兜铃酸 关木通 炮制工艺 

分 类 号：R283[医药卫生—中药学]