固相萃取富集-高效液相色谱法测定大花红景天中微量元素硒  

HPLC Determination of Selenium in Rhodiola Crenulata by Solid Phase Extraction

作　　者：吴少雄[1] 杨式华 杨光宇[3] 郭祀远[1] 李琳[1] 王保兴 

机构地区：[1]华南理工大学轻工与食品学院 [2]云南瑞升科技有限公司,昆明650223 [3]云南烟草科学研究院,昆明650223

出　　处：《食品与发酵工业》2005年第7期107-109,共3页Food and Fermentation Industries

基　　金：广州市科技攻关项目(No.2002Z3-E0321)

摘　　要：研究了用固相萃取富集,二极管矩阵检测器检测,反相液相色谱法测定大花红景天中微量元素硒的方法。样品中硒用邻苯二胺显色剂作柱前衍生,WatersSep-park-C18固相萃取微柱富集硒的邻苯二胺络合物,然后用乙腈和甲醇为流动相,WatersμBondapakC18色谱柱为固定相分离,用二极管矩阵检测器检测。方法相对标准偏差为2·40%,标准回收率为98·67%。该方法用于大花红景天样品的分析,结果令人满意。The method for the determination of selenium in health food materials by solid phase extraction-high performance liquid chromatography was studied. The selenium was pre-column derived by o-phenylendiamine. The chelate complex can be extracted by Waters Sep-Park-C18 chromatogram column and eluted by acetonitrile, then separated on a Waters μBondapak C18 chromatogram column（3.9mm×300 mm） and monitored by photodiode array detector. The relative standard deviation is 2.40%, and the recovery is 98.67% . This method can be applied to the determination of microamount of selenium in Rhodiola Crenulata with satisfactory results.

关 键 词：邻苯二胺 固相萃取富集 高效液相色谱法 硒 微量元素硒 大花红景天 测定 反相液相色谱法 相对标准偏差 标准回收率 

分 类 号：O657.72[理学—分析化学] X131.2[理学—化学]