外周血CD_4^+CD_(25)^+T细胞表达在系统性红斑狼疮患者中的意义  

Clinical significance of CD_4^+CD_(25)^+ T cells in peripheral blood of patients in systemic lupus erythematosus

作　　者：杨旭燕[1] 鲁晓勇[1] 许东航[1] 吕庆华[1] 王巧宏[1] 吴华香[1] 

机构地区：[1]浙江大学医学院附属第二医院风湿科,310009

出　　处：《中华内科杂志》2005年第8期570-572,共3页Chinese Journal of Internal Medicine

基　　金：浙江省自然科学基金(Y204431);浙江省教育厅基金(20040208)资助项目

摘　　要：目的了解系统性红斑狼疮(SLE)患者外周血CD4+CD2+5T细胞表达及其临床意义。方法用流式细胞仪检测53例SLE患者外周血CD4+CD2+5T细胞表达,根据CD25表达荧光强度>100者为CD4+CD25brightT细胞,并与SLE活动指数评分(SLEDAI)、血清补体C3水平、抗双链DNA抗体、抗核抗体滴度进行相关分析。结果SLE患者外周血CD4+CD2+5T细胞表达率为(7·84±1·85)%,显著低于对照组(9·18±2·01)%(P<0·05),且活动期组[(6·72±1·16)%]较稳定期组更低[(8·57±1·91)%,P<0·01]。外周血CD4+CD25brightT细胞表达率在活动期组[(0·85±0·24)%]和稳定期组[(0·91±0·25)%]之间相比差异无统计学意义(P=0·686),但均低于对照组[(1·43±1·08)%,P<0·01]。随治疗后SLEDAI评分的下降,活动期组SLE患者外周血CD4+CD25brightT细胞表达率无明显变化。进一步分析外周血CD4+CD25brightT细胞表达率与SLEDAI评分、抗核抗体、抗双链DNA抗体滴度和血清C3水平的相关性,分别为ρ=-0·188,P=0·178;ρ=-0·216,P=0·121;ρ=0·082,P=0·560;ρ=0·010,P=0·944,差异均无统计学意义(P>0·05)。结论外周血CD4+CD2+5T细胞的减少可能与SLE的发病有关。Objective To investigate the expressions of CD4^＋CD25^＋T cells in the peripheral blood of patients with systemic lupus erythematosus （SLE） and to identify the relationship between the levels of CD4^＋CD25^＋T cells and the disease activity and progression of SLE. Methods Fifty-three SLE patients were enrolled in the study. Flow-cytometric assay was employed for detection of CD^CD2~ T cells and CD4^＋CD25^brightT cells were defined according to fluorescence intensity of CD2s expression exceeding 100. Meanwhile, correlation analysis was performed between their expression and the scores of SLE disease active index （SLEDAI） , C3 , dsDNA and antinuclear antibody titles. Results The levels of peripheral blood CD4^＋CD25^＋T cells in SEE were （ 7.84±1.85）%, which were significantly lower than those in a group of healthy control [ （9.18±2.01） %, P 〈 0.05 ]. The levels of CD4^＋CD25^＋T cells in a group of active SLE [ （6.72±1.16）%] were higher than those in a group of stable SLE [ （8.57±1.91）%, P〈0.01]. There was no difference between the active and stable groups of SEE in peripheral blood CD4^＋CD25^brightT cells [ （0.85±0.24）% vs （0.91±0.25）%, P=0.686], but they were significantly lower than those in the group of healthy controls [ （1.43±1.08） %, P〈0.01 ]. With the reduction of the SLEDAI scores in SLE patients after relevant treatment, the levels of peripheral blood CD4^＋CD25^bright T cells did not change. No correlation was found between the levels of CD4^＋CD25^bright T cells and SLEDAI scores, antinuclear antibody titles, dsDNA and C3, respectively （ρ=-0.188, P=0.178;ρ=-0. 216, P=0.121;ρ=0. 082, P=0.560;ρ=0.010, P=0.944）. Conclusion The reduction of CD4^＋CD25^＋T cells in peripheral blood may participate in the pathogenesis of SLE.

关 键 词：红斑狼疮 系统性 T淋巴细胞 系统性红斑狼疮患者 T细胞表达 CD4^+CD25^+T细胞 外周血 抗双链DNA抗体 SLE患者 CD25^+细胞 SLEDAI 

分 类 号：R593.241[医药卫生—内科学]