杆状病毒介导内皮抑素抗兔动脉粥样硬化作用  

Antiatherosclerotic Effect of Baculovirus-Mediated Endostatin in Rabbits

作　　者：尤蓓[1] 李彪[2] 沈卫峰[1] 陆林[1] 尹桂芝[1] 张一帆[3] 赵龙[3] 贾世海[2] 于金德[1] 

机构地区：[1]上海第二医科大学瑞金医院心脏科 [2]中国科学院上海生命科学研究院植物生理生态研究所 [3]上海第二医科大学瑞金医院核医学科,上海200025

出　　处：《上海第二医科大学学报》2005年第8期776-779,共4页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：上海市科学技术发展基金(014119093)资助项目.

摘　　要：目的研究分泌型重组杆状病毒介导内皮抑素体内抗动脉粥样硬化的作用。方法采用髂动脉内膜剥脱联合高脂喂养法建立兔髂动脉粥样硬化模型。造模后第2周,对照组和实验组分别肌肉注射空载杆状病毒rBacGFP和携带人内皮抑素基因的分泌型重组杆状病毒rBachEndo各1×109pfu,比较治疗前后肝肾功能变化。于治疗2周后处死动物,取病变部位血管观察其病理学改变。结果肝肾功能检测结果表明,rBachEndo治疗未发生肝肾毒性反应;组织学观察显示,治疗2周后,病变血管内膜增厚和管腔狭窄程度较对照组显著减轻,且内膜新生血管明显减少。结论分泌型重组杆状病毒rBachEndo通过介导兔体内骨骼肌分泌内皮抑素,有效抑制动脉粥样硬化斑块生长,且安全性高。Objective To investigate antiatherosclerotic effect in vivo of endostatin mediated by secretory recombinant baculovirus. Methods Intima denudation and lipid-rich breeding were utilized to develop atherosclerotic rabbits. Two weeks after injury, the control group and treated group were given intramuscularly 1×10^9 pfu vehicle rBac-GFP and secretory recombinant baculovirus rBac-hEndo （ which carried the human endostatin gene） , respectively. The hepatic and renal functions of the rabbits were monitored. Two weeks later, all the injured vessels were excised and examined. Results There was no hepatic and renal toxicity observed in the rBac-hEndo treatment. Two weeks after treatment, the thickness of intima and stenosis of the atherosclerotic vessels in the rBac-hEndo treated rabbits were reduced significantly and intimal capillary formation was less than that in the vehicle-treated ones. Conclusion Endostatin secreted in vivo by the skeletal muscle transduced by secretory recombinant baculovirus could inhibit effectively the development of atherosclerotic plaque formation, and this method is highly safe.

关 键 词：杆状病毒 内皮抑素 动脉粥样硬化 

分 类 号：R543.5[医药卫生—心血管疾病]