中药材及中成药中马兜铃酸类成分的定性、定量分析  

Qualitative and quantitative analysis of aristolochic acids in Chinese materia medica and traditional Chinese patent medicines

作　　者：刘丽芳[1] 曹素萍[1] 及莹[1] 张小俊[1] 黄婷[1] 

机构地区：[1]中国药科大学,江苏南京210038

出　　处：《中成药》2005年第8期938-941,共4页Chinese Traditional Patent Medicine

摘　　要：目的:建立马兜铃酸的定性鉴别和含量测定方法。方法:TLC:样品经适当提取、净化后点于硅胶GF254板上,以甲苯醋酸乙酯水甲酸(20∶10∶1∶1)上层为展开剂。色谱条件:流动相:甲醇与1.0醋酸进行梯度洗脱,甲醇在15min内由40到100;检测波长:310nm;流速:1.0mL/min;柱温:40℃。结果:TLC:马兜铃酸Ⅰ的Rf值为0.50,马兜铃酸Ⅱ的Rf值为0.53。HPLC:马兜铃酸Ⅰ的平均回收率为103.5,RSD=0.3;马兜铃酸Ⅱ的平均回收率为96.05,RSD=1.3。结论:本法简单、快速、重现性好。AIM： To establish identification and determination methods for aristolochic acids. METHODS： The extracts of the sample were developed on silica gel GF254 plate, using supernatant layer of the mixture of tolueneethyl acetate-water-formic acid （20 ： 10 ： 1 ： 1 ） as the mobile phase. Chromatogram system：gradient elution with mobile phase consisting of （A） 1% acetic acid in water and （B） methanol was used. The initial condition was at 40% of B and gradient up to 100% B in 15 minutes before returning to the initial conditions. Detection was at 310 nm. The column temperature was set at 40℃ and flow-rate was set at 1.0 mL/min. RESULTS： TLC：the Rf value of aristolochic acid Ⅰ was 0.50 and aristolochic acid Ⅱ was 0.53. The results of assay were shown as follow ： The average recovery of aristolochic acid Ⅰ was 103.3% （RSD = 0.98% ）. The average recovery of aristolochic acid Ⅱ was 95.97% （RSD = 1.2% ）. CONCLUSION： It reveals that the methods we described are accurate, rapid and reproducible.

关 键 词：马兜铃酸 薄层色谱 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]