用杂交F_1小鼠制备单克隆抗体腹水的研究  

Use of first generation hybrid mice(F_1) in production of ascitic monoclonal antibodies

作　　者：祝庆余 蒋越 李钟铎 耿志贤[1] 江其辉[1] 刘宝兴[1] 

机构地区：[1]军事医学科学院微生物学流行病学研究所,军事医学科学院实验动物中心

出　　处：《军事医学科学院院刊》1995年第3期175-179,共5页Bulletin of the Academy of Military Medical Sciences

摘　　要：用ＢＡＬＢ／ｃ×ＬＡＣＡＦ＿１和ＢＡＬＢ／ｃ×Ｃ＿（５７）Ｆ＿１杂交小鼠与纯系ＢＡＬＢ／ｃ小鼠生产腹水制备单克隆抗体，观察各种小鼠对杂交瘤细胞的耐受性，产生腹水的能力及腹水中蛋白质含量和抗体效价。ＢＡＬＢ／ｃ×ＬＡＣＡＦ＿１鼠的平均腹水产量，雄鼠为ＢＡＬＢ／ｃ鼠的２～３倍，雌鼠为１．４倍左右。对杂交瘤细胞的耐受性，腹水中蛋白质含量及抗体效价与ＢＡＬＢ／ｃ小鼠比较均无显著差异。ＢＡＬＢ／ｃ×Ｃ＿（５７）Ｆ＿１小鼠的腹水产量明显低于ＢＡＬＢ／ｃ小鼠，而且死亡率较高。ＢＡＬＢ／ｃ×ＬＡＣＡＦ＿１小鼠生长快，易于饲养繁殖，价格便宜，腹水产量高，是一种替代ＢＡＬＢ／ｃ小鼠生产单克隆抗体腹水较理想的鼠种。The ability of producing ascitic monoclonal antibodies of the first generation hybridmice,BALB / c × LACA and BALB / c × C_(57),were compared with that of BALB / c parents.Ascitic fluids produced in the hybrid F_1 and BALB / c mice were evaluated in terms of volume,antibody titers and protein levels.The results indicated that BALB / c × LACA F_1 producedsignificantly more ascitic fluid of equally high antibody titers and protein levels compared to theBALB / c parent, approximately 1.4 times more for the females and 2-3 times more for the males.BALB/c×LACA F_1 grow faster and cost less than the BALB/c.It is suggested that BALB/c×LACA F_1 mice may be used in production of ascitic monoclonal antibodies instead of BALB/c mice.

关 键 词：单克隆抗体 杂交瘤 腹水 小鼠 制备 

分 类 号：R392-33[医药卫生—免疫学]