紫花苜蓿根原生质体植株再生  

Plant Regeneration from Root Protoplasts of Medicago Sativa

作　　者：黄绍兴[1] 王慧中[1] 吕德扬[1] 

机构地区：[1]北京农业大学生物学院,杭州师范学院生物系,中国科学院遗传研究所

出　　处：《科技通报》1995年第4期232-234,共3页Bulletin of Science and Technology

摘　　要：用三种酶液游离大量高活力的紫花苜蓿实生苗根原生质体，Ｋａｏ的原生质体培养基使根原生质体分裂形成细胞团。原生质体形成的愈伤组织在ＵＭ培养基上分化体细胞胚。体细胞胚在无激素的ＭＳ琼脂培养基上发育为完整植株。Protoplasts were released from roots of seedling of Medicago sativa by three enzyme solutions.Root pro-toplasts of Medicago sativa divded to form colonies(small calli)in Kao' s protoplast cultuer medium. Proto-plast-derived calli developed somatic embryos on UM medium. Somatic embryos developed into whole plantson hormone free MS medium.

关 键 词：紫花苜蓿 原生质体 植株再生 豆科 牧草 

分 类 号：S541.035.3[农业科学—作物学]