RAPD技术的特点及其在昆虫分类中的应用  

THE PECULIARITY OF RAPD AND ITS APPLICATION TO INSECT TAXONOMY

作　　者：鲁亮[1] 归鸿[1] 

机构地区：[1]南京师范大学生物系

出　　处：《昆虫学报》1995年第1期117-122,共6页Acta Entomologica Sinica

摘　　要：随机扩增的多态性ＤＮＡ（ＲＡＰＤ）技术，是近年来发展起来的一项ＤＮＡ分子水平上的大分子多态检测手段。由于它具有简捷、灵敏、对材料要求不高、取材少、成本低等优点，备受人们青睐，在遗传学、分子进化、生物分类等领域被广泛地运用。在昆虫分类的过程中，由于昆虫的种类繁多，形态、生态差异很大，许多在其它领域被广泛运用的分子生物学技术不能在昆虫分类中充分发挥作用。同时在昆虫分类中出现的一些问题却又需要用涉及遗传本质的分子生物学技术进行探讨和研究。Random Amplified Polymorphic DNA (RAPD), which has been developing since 1990, is a molecular approach to reveal DNA polymorphism. It is believed that this approach is valuable in studies involving genetics, molecular evolution, taxonomy et al. because of its advantages, such as simplicity, sensitivity, cheapness and, the most important, lower requirement of materials.In the field of insect taxonomy, the molecular approaches which are valuablein other fields can not be used widely due to polymorphism of insects. The obstacle to the progress of insect taxonomy should better be overcome by molecular approaches which relate to DNA. Now, RAPD is considered to be an effective key.In this paper, the peculiarity of RAPD and its superiority in insect taxonomy are discussed.

关 键 词：随机扩增的多态性DNA 昆虫分类 

分 类 号：Q969[生物学—昆虫学]