不同光学异构体组成的氯氰菊酯对敏感及抗性家蝇的神经电生理学研究  

AN ELECTROPHYSIOLOGICAL STUDY ON THE RESISTANT HOUSEFLIES TREATED WITH DIFFERENT OPTICAL ISOMERS OF CYPERMETHRIN

作　　者：张文吉[1] 钱洪[1] 程会文[1] 周培爱[1] 

机构地区：[1]北京农业大学应用化学系,北京大学生物学系,中国农业科学院蔬菜花卉研究所

出　　处：《昆虫学报》1995年第3期257-265,共9页Acta Entomologica Sinica

基　　金：国家自然科学基金

摘　　要：采用敏感家蝇（ＭｕｓｃａｄｏｍｅｓｔｉｃａｖｉｃｉｎａＬ．）及由５种不同光学异构体组成的氯氰菊酯选育的抗性家蝇中胸足离体标本，观测五种药剂对足感觉神经纤维冲动发放的影响。各种异构体组成的氯氰菊酯均可引起感觉神经纤维发放的增加，然后逐渐降低，直至完全阻断。以阻断时间和加药剂量为参数，求出神经敏感度。结果表明：五种药剂作用于敏感家蝇的神经敏感度与室内生物测定的ＬＤ５０值（μｇ／头）无相关性，而与供试药剂中反α体与顺α体的比例有关，反α体所占比例越多的药物，神经敏感度越高。抗性家蝇的神经敏感度与敏感家蝇相比大幅度下降，可以认为神经敏感度降低是家蝇对氯氟菊酯产生抗性的主要机制。抗性家蝇中，氯氰菊酯品系（ＲＣ１）的抗生水平最高，但它的神经敏感度较其它抗性品系也高，由此推测，ＲＣ１的抗性机制与其它品系有所不同。A neurophysiological assay was conducted to investigate the effects of five mixtures of cypermethrin stereoisomers on firing of the sensory nerve in the isolated mesathoracic leg of susceptible and resistant houseflies Each mixture induced an elevation of the firing frequency, then gradual drop and eventually to blockade. Nerve sensitivity was calculated by the blocking time and cyermethrin doses. The sensitivity of the susceptible strain toward five mixtures of cypermethrin stereoisomers was in the order C4 > C1 > C2 > C3 > C5. The sensitivity was dependent on the ratio of trans- to ets-isomer in the mixtures, the higher the ratio the higher the sensitivity. The results showed that the nerve sensitivity of resistant houseflies decreased markedly. It is suggested that the decrease of nerve sensitivity might be the most important mechanism of resistance in the houseflies to cypermethrin. The resistance levels of RC, was the highest among the resistant house fly strains, but its neurosensitivity was also the highest. It was inferred that the main resistance mechanism of RC, to cypermethrin is different from that of other strains.

关 键 词：光学异构体 氯氰菊酯 家蝇 神经电生理 抗性 

分 类 号：S482.32[农业科学—农药学] Q969.453.1[农业科学—植物保护]