裂叶悬钩子器官发生的细胞组织学观察  

CYTO-HISTOLOGICAL OBSERVATION ON ORGANOGENESIS OF RUBUS LACINIATUS CULTURE IN VITRO

作　　者：柯善强 傅俊 桂耀林[2] 徐廷玉[2] 

机构地区：[1]中国科学院武汉植物研究所,武汉430074 [2]中国科学院植物研究所,北京100044

出　　处：《Acta Botanica Sinica》1989年第11期889-891,共3页Acta Botanica Sinica（植物学报：英文版）

基　　金：中国科学院资助课题

摘　　要：裂叶悬钩子(Rubus laciniatus W.cv.Thornless Evergreen)是蔷薇科悬钩子属一种重要的小果类植物。我们在以离体叶片诱导不定芽成功的基础上,对其叶片不定芽形态发生过程作了进一步观察。供接种的叶片从基部去掉叶柄,按前文的操作顺序进行灭菌,接种和培养。从培养第3天起,从叶片基部与叶柄接合处切取材料固定,以后每隔2天取样一次。Leaf explants of Rubus laciniatus were cultured on Nitsch and Nitsch basic medium supplemented with either 6-BA 2 mg/L and NAA 0.1 mg/l or 2,4-D 2.5 mg/l and NAA 0.1 mg/l.Adventitious buds were produced from leaf-like structures. Some ultrastructural observations of cells have shown that the most easy change part of a plastid during the cell differentiation in Rubus leaf explant culture in vitro was lamella system,storage starch and plastogluboli.

关 键 词：悬钩子 器官发生 组织学 

分 类 号：Q944.6[生物学—植物学]