马尾松离体培养条件下的微繁殖和菌根的形成  

Micropropagation and Mycorrhizae Formation of pinus massoniana Lamb.in vitro

作　　者：成小飞[1] 花晓梅[1] 李文钿[1] 

机构地区：[1]中国林业科学研究院森林生态环境研究所,中国林业科学研究院林业研究所

出　　处：《林业科学研究》1995年第3期241-246,共6页Forest Research

基　　金："八五"攻关课题

摘　　要：以马尾松种胚作外植体进行不定芽诱导。基本培养基的种类对外植体不定芽的诱导起着决定性的作用，在ＧＤ附加２．０ｍｇ／ＬＢＡ的培养基上，外植体不定芽诱导率达７０％以上。ＩＡＡ或ＮＡＡ的加入不利于外植体不定芽的诱导。在不定芽生长培养基中，加入适量的ＩＢＡ能促进不定芽的伸长生长。伸长至０．５ｃｍ以上的嫩枝在１／２ＧＤ附加４．０ｍｇ／ＬＩＢＡ的培养基上，生根状况良好。生根后的小植株，在１／２ＧＤ培养基上接种Ｒｔ４９菌根真菌，菌丝侵染进入根部，形成组培菌根植株。The mature embryos of Pinus massoniana were used as explants to induce ad-ventitious buds on GD、SH、MS or MCM medium supplemented with 0.5～4.0 mg/LBA and0.0～1.0 mg/L IAA. It has been showed that the basic medium played a determinative rolein initiating the adventitious buds. The optimal combination of the medium was GD + 2.0mg/LBA without IAA,on which over seventy percent of explants was induced.The additionof IAA or NAA was disadvantageous to the formation of adventitious buds. By adding opti-mal concentration of IBA in the growth medium,the elongation of the adventitous buds wasimproved.The elongated shoots were induced to produce adventitious roots on 1/2 GD medi-um supplemented with 4.0mg/LIB Both the plantlets and mycorrhizal fungi Rt49 wereinoculated on l/2 GD medium simultaneously. The mycorrhizal fungi infected the root tipsand then mycorrhizae formed.

关 键 词：马尾松 离体培养 菌根 

分 类 号：S791.248[农业科学—林木遗传育种]