麦胚凝集素亲和层析法分离碱性磷酸酶肝型同工酶及其临床意义  

WHEAT GERM AGGLUTININ AFFINITY CHROMATOGRAPHY FOR SEPARATING LIVER ALKALINE PHOSPHATASE IN SERUM AND ITS CLINICAL SIGNIFICANCE

作　　者：吕晴[1] 段恕诚[1] 朱启镕[1] 陈惠黎[1] 吕元[1] 陈敏[1] 徐华芳[1] 

机构地区：[1]上海医科大学儿科医院,上海市卫生防疫站食品监测所

出　　处：《临床肝胆病杂志》1995年第1期23-24,共2页Journal of Clinical Hepatology

摘　　要：应用麦胚凝集素亲和柱层析分离血清碱性磷酸酶肝型同工酶，正常小儿为２５．０±５．９（％），急性肝炎患儿为６３．４±５．７（％），慢性肝炎为３２．５±８．１（％），肝炎后肝硬化为４０．２％，均明显升高。本方法分离效果好，采用荧光比色法检测碱性磷酸酶活性，可检出总酶活力在正常范围而肝型同工酶已有异常的病例，使诊断更具敏感性和特异性。Abstract We used lectin affinity chromatography to separate liver alkaline phosphatase (L-ALP)in serum from children. The results are as follow : The normal range is 25. 0± 5. 9 (%). The range of acute hepatitis, 63. 4±5. 7 (%). The range of chronic hepatitis, 32. 5±8. 1 (%)and liver cirrhosis is 40. 2 (%). The above results show that the value of L-ALP of children with liver disease are more higher than that of normal children. This procedure has effective capacity for finding those patients whose total ALP activity still falls within the normal range but L-ALP has apparently increased, which inakes the diagnosis more specific. Another Significance of this work is that it has laid thefoundation for further analysis of the structure of sugar chain of L-ALP.

关 键 词：碱性磷酸酶 同工酶 麦胚凝集素 肝炎 病毒性肝炎 

分 类 号：R512.604[医药卫生—内科学] R575.104[医药卫生—临床医学]