全血2，3－二磷酸甘油酸比色测定法  

Colorimetric determination of 2,3-diphosphoglyceric Acid in blood by phosphoglycerate mutase

作　　者：顾光煜[1] 王以立[1] 任强 常福久 薛永 

机构地区：[1]南京市鼓楼医院检验科,江苏省张家港市中医院,南京市六合县中医院

出　　处：《临床检验杂志》1995年第5期236-238,共3页Chinese Journal of Clinical Laboratory Science

摘　　要：本文介绍了用磷酸甘油酸变位酶测定２，３－二磷酸甘油酸的比色法。探讨了该法的最适反应条件，最适ｐＨ７．５～７．６。线性范围可达６．０ｍｍｏｌ／Ｌ。三份不同浓度标本批内ＣＶ１．１％～２．１％，批间ＣＶ２．５％～３．９％。６０例南京地区健康成人２．３－二磷酸甘油酸含量为１．６～２．７ｍｍｏｌ／Ｌ，平均回收率９４％，与Ｓｉｇｍａ试剂盒比较测定结果无显著性差异（Ｐ＞０．０５），相关性良好，ｒ＝０．９８２。In this paper.We reported a colorimetric determination for blood 2,3-Diphosphoglyceric Acid(2,3-DPG) by phosphoglycerate mutase(PGM),We studied optimum conditions of this method and optimum pH was 7.5～7.6.The liner range was up to 6.0 mmol/L. The coefficient of variation of three samples in vary concentration was 1. 1%～2. 1% intr-assay and 2.5%～3.9% inter-assay.The reference value in blood 2, 3-DPG of 60normal adults in Nanjing area was 1. 6～2. 7 mmol/L. The mean recovery rate was 94%. The results estimated by our kits correlated closely with those obtained by Sigma kits and the correlation coefficients was r=0. 982. This method is rapid, simple and accurate. The regent had a long term stability and was easy to buy and was convenient In use.

关 键 词：二磷酸甘油酸 比色法 全血 

分 类 号：R446.112[医药卫生—诊断学]