供肾灌注液中酶学改变的实验研究  

Laboratory study of enzymatic activity in infusion solution of pretransplanted graft

作　　者：林荣禧[1] 欧良明[1] 陈子宣[1] 郑智勇[1] 洪佳平[1] 林文洪[1] 赵明[1] 吴卫真[1] 

机构地区：[1]南京军区福州总医院泌尿外科

出　　处：《临床泌尿外科杂志》1995年第1期46-47,共2页Journal of Clinical Urology

摘　　要：用上海中心血站研制的肾保存液（ＨＣＡ）保存的６３例６３个供肾（分成Ａ、Ｂ、Ｃ三组）在不同的缺血时间，观察其灌注液中Ｎ－乙酰β－葡萄糖苷酶（ＮＡＧ）及乳酸脱氢酶（ＬＤＨ）的浓度改变，并对缺血２４ｈ以上的１０个供肾（Ｂ、Ｃ组）作了病变程度半定量分析。结果表明，缺血２４ｈ和１２ｈ以下的供肾，其灌注液中ＮＡＧ及ＬＤＨ改变无明显差异，而缺血４８ｈ以上的供肾，ＮＡＧ及ＬＤＨ均升高，但其中ＮＡＧ升高非常明显（Ｐ＜０．００１）；病变程度半定量分析亦显示缺血４８ｈ后供肾病变程度亦明显加重（Ｐ＜０．００１）。因此，灌注液中ＮＡＧ测定可以作为供肾质量的一个重要参数。Abstract Author investigated activity of NAG and LDH in infusion solution of pretransplanted graft with different ischemic time preserved using HCA solution produced by Shanghai Central Blood Bank, and performed semiquantitch tive analysis of pathological lesion for the grafts with more than 24 hours ischemic time. The results indicated that NAG and LDH in the solution of the grafts with less than 12 hours and 24m hours ischemic time were no diffrence(P>0.5)and in the grafts with more than 48 hours they were increased. NAG was heighter(P<0.001).The semiquantitive analysisof pathological lesion indicated that the lesion of the grafts with more than 48 hours ischemic time were heavy (P<0.001). Author considered that NAG in the HCA solution may be a important paramater for assessment of graft quality.

关 键 词：移植肾 灌注液 葡萄糖苷酶 乳酸脱氢酶 

分 类 号：R699.2[医药卫生—泌尿科学]