病毒性心肌炎心内膜心肌活检石蜡包埋标本中巨细胞病毒基因的原位多聚酶链反应  

The detection of cytomegalovirus genes in paraffin embedded endomyocardial biopsy samples of viral myocarditis by in situ polymerase chain reaction

作　　者：李武[1] 杨英珍[1] 陈灏珠[1] 

机构地区：[1]上海医科大学中山医院,上海市心血管病研究所

出　　处：《临床心血管病杂志》1995年第6期327-329,共3页Journal of Clinical Cardiology

基　　金：美国中华医学基金

摘　　要：对２５例病毒性心肌炎（ＶＭＣ）心内膜心肌活检（ＥＭＢ）石蜡包埋标本，用原位多聚酶链反应（ＩＳＰＣＲ）方法进行了巨细胞病毒（ＣＭＶ）的原位检测，心肌细胞内阳性检出率为４４％，明显高于对照组（Ｐ＜０．０１），但病毒基因与心肌病理学改变的关系尚不能确定。提示在心肌内ＣＭＶ基因检测中，ＩＳＰＣＲ是一种迅速、敏感和特异的方法，与原位杂交方法相比，其阳性检出率虽略有提高，但差异不具有统计学意义。Abstract The cytomegalovirus genes were detected by in situ polymerase chain reaction(ISPCR) in endomyocardial biopsy(EMB) specimens from 25 patients with viral myocarditis(VMC). The paraffin embedded sections and hot-start technique were used in this detection. The positive signals were found in cardiomyocytes of 11 patients(44%), which was significantly higher than that of control group(P<0.01). The results suggest that the ISPCR is a sensitive and specific method to detect CMV-DNA in EMB samples, Compared with in situ hybridization, the positive rate of detection by ISPCR was slightly increased but there was no statistically significant difference between these two methods.

关 键 词：心肌炎 巨细胞病毒 病毒性心肌炎 多聚酶链反应 

分 类 号：R542.21[医药卫生—心血管疾病]