微波固定对琥珀酸脱氢酶细胞化学影响的电镜观察  

Electron microscopic observation of cytochemical effect of microwave fixing on succinodehydrogenase

作　　者：胡闻[1] 肖莎[1] 汪凤霞[1] 

机构地区：[1]安徽省立医院病理科,第一军医大学珠江医院病理科,安徽省合肥市第一人民医院

出　　处：《临床与实验病理学杂志》1995年第2期105-107,共3页Chinese Journal of Clinical and Experimental Pathology

摘　　要：对浸泡在四种不同液体（生理盐水、０．２５％戊二醛、２．５％戊二醛、５％戊二醛）中的鼠肝组织进行微波照射固定，并按琥珀酸脱氢酶（ＳＤＨ）常规孵育、制片、电镜观察。结果表明：四个组都随微波照射时间增加，液体温度不断升高，肝细胞内线粒体ＳＤＨ酶活性由强逐渐减弱，定位趋于稳定；其中生理盐水ＳＤＨ酶活性保存最好，且以微波照射４０ｓ时，ＳＤＨ酶活性及定位最佳，超微结构清晰。Liver tissues of rat in four different kinds of liquid(normal saline,0. 25% GLA,2.5%GLA,5%GLA)were fixed by mesns of microwave irradiation. Incubation, process and observation were made according to succinodehydrogenase(SDH)routine. The results indicated that the four groups shared the same rule that with the increase of irradiation time and the liquid temperature raised continuallg, the activity of SDH in mitochondrion of liver cells decreased,but the localization become stabilized.The activity of SDH was best preserved in normal saline group, better than any other GLA group, and when irradiation time was 40 s,in the same group we got the best activity and localization of SDH,and the ultrastructure was very clear.

关 键 词：微波照射 肝细胞 琥珀酸脱氢酶 组织化学 电镜 

分 类 号：Q554.3[生物学—生物化学]