维持液中小牛血清浓度对LDH法检测NK活性的影响  

The NK Cytotoxic Index Influnced by the FCS Concentration Supple- mented in Maintenance Medium in Measurement by LDH Realease

作　　者：汪翠玲[1] 全家妩[1] 

机构地区：[1]卫生部武汉生物制品研究所

出　　处：《免疫学杂志》1995年第3期190-193,共4页Immunological Journal

摘　　要：本文对含不同浓度小牛血清（ＦＣＳ）维持液中靶细胞的自然释放、最大释放及效应细胞释放进行检测，结果表明：效应细胞自然释放随ＦＣＳ浓度增加而增加显著，当ＦＣＳ为２０％时，其释放接近甚至超过最大释放；ＦＣＳ低于５％时，其释放趋于稳定。由结果看，无ＦＣＳ时，各项释放最稳定，但经相关性试验检测，无ＦＣＳ维持液检测结果与含ＦＣＳ的维持液检测结果不相关。The max releases,spontaneous releaxes and the release,of effector cells in maintenance medi- ums varied with FCS concentration were measured in this paper. The results showed that releases of effectorcells increased significantly by the increase of FCS concentration supplemented in the maintenance mediums,and it was near or more than the maximal releases of target cells when the FCS concentration was 20%,Eachkind of the releases was more stable and the releases of effector cells approached the spontaneous releases oftarget cells when the FCS concentration was less than 5%. According to our experiments, the maintenancemedium without FCS in best for the measurements,but the results of reliability study indicated the assay inmaintenance medium without FCS was not comparable with the assaay in ones with FCS.

关 键 词：靶细胞 效应细胞 LDH 细胞毒试验 自然杀伤细胞 

分 类 号：R392.12[医药卫生—免疫学] R446.61[医药卫生—基础医学]