氯化铜对滇紫草细胞色素形成的影响  

EFFECT OF CuCl_2 ON SHIKONIN DERIVATIVES FORMATION IN ONOSMA PANICULATUM CELL CULTURES

作　　者：宁文[1] 曹日强[1] 

机构地区：[1]南京大学生物系

出　　处：《南京大学学报（自然科学版）》1995年第2期334-337,共4页Journal of Nanjing University（Natural Science）

摘　　要：滇紫草细胞于Ｍ－９培养液中悬浮培养以形成紫草色素。当Ｍ－９培养液中ＣｕＳＯ４·５Ｈ２０由相同浓度的ＣｕＣｌ２·２Ｈ２Ｏ取代后，色素产量比对照提高了５８．７％，ＣｕＣｌ２·２Ｈ２Ｏ的最适浓度为３０μｍｏｌ／Ｌ。滇紫草细胞的继代培养基Ｂ—５中的ＣｕＳＯ４·５Ｈ２Ｏ取代后，培养所得的细胞接种于Ｍ－９培养液中，色素产量比对照提高了３２．０８％，ＣｕＣｌ２·２Ｈ２Ｏ的最适浓度为０．６μｍｏｌ／Ｌ．ＣｕＣｌ２·２Ｈ２Ｏ对细胞生长无不良影响。Ｍ—９培养液中，铜于色素形成的初期，可快速吸附于细胞上，这一现象为ＣｕＣｌ２·２Ｈ２Ｏ促进紫草色素合成机制的深入研究提供参考。Cu2+ strongly stimulated the biosynthesis of shikonin derivatives. Onosma paniculatum cells were inoculated in M-9 medium to produce shikonin derivatives. When CuSO4 5H2O of M-9 was substituded by Cucl2. 2H2O of the same concentration (1. 2μmol/L),the shikonin production was higher than that of the control (increased 58. 7 % ). The optimum concentration of CuCl2. 2H2O was 30μmol/L. Onosma cells were subcultured on B-5 medium. When CuSO4. 5H2O of B-5 medium was substituded by CuCl2. 2H2O of the same concentration (0. 1μmol/L), the cells' shikonin production in M-9 medium increased by 32. 08%. The optimum concentration of CuCl2. 2H2O was 0. 6μmol/L. CuCl2. 2H2O had nonegative effect on cells growth. During the early stage of shikonin derivatives fromation, Cu2+ in M-9 medium might be absorbed quickly in cells. This phenomenon suggests the reference for the study on the mechanism of CuCl2's stimulating the biosynthesis of shikonin derivatives.

关 键 词：细胞培养 紫草色素 氯化铜 紫草科 

分 类 号：Q949.777.4[生物学—植物学]