聚合酶链反应检测淋球菌的临床应用  

CLINICAL APPLICATION OF DETECTION OF NEISSERIA GONORRHOEAE BY POLYMERASE CHAIN REACTION

作　　者：李明发[1] 项凤青[1] 郑谷[1] 蒋清[1] 戴玉田[1] 熊晓明[1] 单春梅 

机构地区：[1]南京铁道医学院生物学教研室,南京市鼓楼医院泌尿外科,盐城市第一人民医院

出　　处：《南京铁道医学院学报》1995年第4期231-233,共3页Journal of Nanjing Railway Medical College

摘　　要：应用ＰＣＲ法对１１３例临床标本中淋球菌菌体内所特有的４．２ｋｂ隐蔽性质粒上的ｃｐｐＢ基因进行了检测。与直接涂片法相比，ＰＣＲ法的敏感性和特异性分别达１００％和８７．５％。PCR assay was performed on 113 clinical samples to detect the cppB gene of the plasmidwhich is specific for all Neisseria gonorrhoeae and approximately 4.2 kb in size;Comparing withthe direct smear assay,the PCR has a sensitibility of 100%and specificity of 87.5%.

关 键 词：聚合酶链反应 淋病双球菌 检测 淋病 诊断 

分 类 号：R759.204[医药卫生—皮肤病学与性病学]