人胎肝GGT纯化及其抗血清制备  

PURIFICATION OF γ-GLUTAMYL TRANSFERASFERASE FROM HUMAN FETAL LIVER AND PREPERATION OF ITS ANTISERUM

作　　者：黄介飞 姚登福 魏群 张弘 倪润洲 肖明兵 孟宪镛 

出　　处：《南通医学院学报》1995年第2期163-166,共4页ACTA Academiae Medicinae Nantong

基　　金：江苏省应用基础研究计划项目

摘　　要：以生化方法从人胎肝组织中提取及纯化人胎肝ＧＧＴ。用此酶作抗原免疫新西兰兔，满意地制各了兔抗人胎肝ＧＧＴ抗血清。应用火箭免疫电泳酶呈色法，显示此抗血清与人胎肝ＧＧＴ抗原及原发性肝癌病人血清均具有很好的特异性免疫反应。The γ-glutamyl transferase (GGT) of human fetal liver was abstracted and purified from human fetal liver tissure by biochemical method.New Zealand rabbil was immunized using the purified enzyme as antigen. The rabbit antiserum against GGT purified from human fetal liver was prepared satisfactorily.Rocket immunoelectrophoresis with enzyme colouration was applied.It was shown that there were satisfactory and specific immune reactions of this antiserum against both the purified GGT antigen and the serum of patient with primary hepatic carcinoma (PHC).

关 键 词：谷氨酰移换酶 抗血清 制备 免疫检测 肝肿瘤 

分 类 号：R392－33[医药卫生—免疫学]