快速提取临床标本中结核杆菌DNA方法的研究  

A Study on a Rapid Method for Extraction of Mycobacterium Tuberculous DNA from Sputum and Body Fluids

作　　者：姚娟[1] 金庆文[1] 侯熙德[1] 赵忠[1] 王宁山[1] 王东[2] 陈荣[2] 

机构地区：[1]南京医科大学第一附属医院神经科 [2]南京市胸科医院检验科

出　　处：《南京医科大学学报（自然科学版）》1995年第1期52-54,共3页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：介绍了二种快速、简便从临床结核性痰标本及体腔液标本中提取DNA的方法,40例结核性标本PCR扩增结果:加热碱裂解法阳性率为67.5%(27/40);溶菌酶法阳性率是60%(24/40).二种方法经统计学处理,PCR阳性率之间无显著性差异(P>0.05).这些方法中,我们省去了传统的酚拙提及蛋白酶K的作用步骤,使提取DNA能在1小时内完成,适用于临床推广.M. Tuberculous DNA was extracted from sputum and body fluid of pa-tients with tuberculosis by alkali or lysozyme hydrolysis. Of 40 tuberculous specimens, the positive rate revealed by PCR was 67. 5%and 60. 0% respectively. There was no significant difference between these two methods (p>0. 05). The results showed that these two meth-ode without phenol extraction and prothinase K digestion were simple and rapid than the con-ventional method. The whole procedure could be completed within one hour. They are rec-ommended for clinical laboratories.

关 键 词：结核杆菌 DNA 提取 多聚酶链反应 

分 类 号：R446.5[医药卫生—诊断学]